Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:4.1.1.15 (
glutamate decarboxylase
)
2,169
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
An improved method for the determination of
glutamate decarboxylase
(
GAD
) activity is described. The enzyme was evaluated by incubation with glutamic acid (L-Glu) in the presence of pyridoxal 5'-phosphate (PLP): the gamma-aminobutyric acid (GABA) formed was derivatized to
PTC
-GABA; the latter was subsequently separated and assayed by isocratic HPLC (LiChrospher RP-18 column; isocratic elution with pH 5.8 acetate buffer in acetonitrile-water) with UV absorbance detection at 254 nm. The method described is a sensitive, reproducible and specific assay useful for following variations of
GAD
activity in vitro; this assay was subsequently used for the evaluation of
GAD
activity variations after irradiation with low doses of He-Ne laser radiation.
...
PMID:Determination of glutamate decarboxylase by high-performance liquid chromatography. 879 13
