Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.4.4 (kinesin)
 5,033 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

KIF3 is a heterotrimeric member of the kinesin superfamily of microtubule associated motors. This functionally diverse family of motor is involved in anterograde transport of membrane bound organelles in neurons and melanosomes, mediates transport between the endoplasmic reticulum and the Golgi, and transports protein complexes within cilia and flagella required for their morphogenesis. Interestingly, a mutation of KIF3, which impairs ciliogenesis in nodal cells, prevents the unidirectional leftward flow (nodal flow) of putative morphogens during embryogenesis, thereby altering the development of left-right asymmetry in mammals.
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PMID:Stirring up development with the heterotrimeric kinesin KIF3. 1120 56

Mammalian left-right determination is a good example of how multiple cell biological processes coordinate in the formation of a basic body plan, but until recently its mechanism was totally elusive. In the past 10 years, molecular genetic studies of kinesin and dynein motor proteins, live-cell imaging techniques, and theoretical studies of fluid mechanics revealed unexpected mechanisms of left-right determination. The leftward movement of fluid at the ventral node, called nodal flow, is the central process in symmetry breaking on the left-right axis. Nodal flow is autonomously generated by the rotation of posteriorly tilted cilia that are built by transport via the KIF3 motor on cells of the ventral node. Recent evidence suggests that nodal flow transports sheathed lipidic particles, called nodal vesicular parcels (NVPs), to the left edge of the node, which results in the activation of the noncanonical Hedgehog signaling pathway, an asymmetric elevation in intracellular Ca(2+), and changes in gene expression. This chapter reviews techniques for the observation of nodal cilia movement and nodal flow in living vertebrate embryos.
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PMID:Observation of nodal cilia movement and measurement of nodal flow. 2040 91

This paper examines the significance of the recent demonstration of polar auxin transport (PAT) in the green macroalga Chara (Charophyceae: Charales) and, especially, options for explaining some features of PAT in the Charales. The occurrence of PAT in the Charales shows that PAT originated in the algal ancestors of the embryophytes (liverworts, mosses, hornworts, and vascular plants), although it is not yet known if PAT occurs elsewhere in the Charophyceae or in other algae. While in the embryophytes PAT occurs in parenchymatously constructed structures which commonly also have xylem and phloem (or their bryophyte analogues) as long-distance transport processes in parallel to PAT, in Chara corallina PAT shares the pathway for long-distance transport of nutrients though the parenchymatously constructed nodal complexes and the single giant cells of the internode. The speed of auxin movement of PAT is much more rapid than that attributable to diffusion and of the same order as the rate of cytoplasmic streaming in the giant internodal cells, yet complete inhibition of streaming by the inhibitor cytochalasin H does not slow down auxin transport. Explanations for this phenomenon are sought in the operation of other mechanochemical motors, dynein-tubulin and kinesin-tubulin, as alternatives to the myosin-actin system which powers cytoplasmic streaming. Experiments in which microtubules are disrupted, for example by colchicine, could show if one of the tubulin-based motors is involved. If these motors are involved, some mechanism is needed to amplify the speeds known for the motors to explain the order of magnitude higher speeds seen for auxin transport.
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PMID:Polar auxin transport in relation to long-distance transport of nutrients in the Charales. 2247 86

Hairy cell leukemia is a rare cancer of the blood. The occurrence of hairy cell leukemia with another very rare genetic disorder makes us question whether it is just a coincidence. This article reports the first case of hairy cell leukemia in a patient with situs inversus totalis in western literature. There have been studies into the pathogenesis of situs inversus totalis that suggest it is caused by the failure of embryonic cells to properly rotate during embryogenesis. On the molecular level, the nodal cilia, which are responsible for embryonic rotation, are built by transport through the KIF3 complex - a kinesin superfamily of molecular motors. The KIF3 complex is also responsible for N-cadherin movement in cells. Furthermore, it is well known that these cell adhesion molecules play an important role in carcinogenesis and its progression. This report attempts to link the rare conditions and propose a possible genetic relationship between the two.
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PMID:Hairy cell leukemia in a patient with situs inversus totalis: an extremely rare combination. 2364 3

Nasopharyngeal carcinoma (NPC) is a common tumor in south China. Kinesin family member 2A (KIF2A) belongs to the kinesin-13 family and is associated with the growth and invasion of a number of different types of human cancer, including ovarian, breast and prostate cancer. The aim of the present study was to evaluate the expression of KIF2A in NPC and explore the relationship between KIF2A and the basic characteristics of 5-8F cells. Immunohistochemistry was performed on tissues from 97 patients with NPC to assess KIF2A protein expression. KIF2A was knocked down by a specific short interfering (si)RNA in 5-8F cell lines. Cell proliferation, apoptosis and cycle were analyzed by MTT assay and flow cytometry. The invasive ability and angiogenesis were evaluated by Matrigel assay and reverse transcription-quantitative PCR. The level of KIF2A was associated with the growth and migration of primary tumor, nodal status and tumor stage. The viability of KIF2A-knockdown cells was decreased compared with that of the control cells. The number of apoptotic cells, as well as the percentage of cells in the G0/G1 phase, was higher in the KIF2A siRNA group compared with the control group. The invasive and angiogenetic ability of 5-8F cells in the KIF2A siRNA group was decreased compared with the control group. In conclusion, the expression of KIF2A correlated with the poor clinicopathological features in NPC. Therefore, KIF2A may serve an important role in the progression of NPC and proliferation of 5-8F cells, which might present a potential therapeutic target for patients with NPC.
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PMID:Effects of KIF2A on the prognosis of nasopharyngeal carcinoma and nasopharyngeal carcinoma cells. 3145 50