Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.4.4 (
kinesin
)
5,033
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
BICD2
is one of the two mammalian homologues of the Drosophila Bicaudal D, an evolutionarily conserved adaptor between microtubule motors and their cargo that was previously shown to link vesicles and mRNP complexes to the dynein motor. Here, we identified a G2-specific role for
BICD2
in the relative positioning of the nucleus and centrosomes in dividing cells. By combining mass spectrometry, biochemical and cell biological approaches, we show that the nuclear pore complex (NPC) component RanBP2 directly binds to
BICD2
and recruits it to NPCs specifically in G2 phase of the cell cycle.
BICD2
, in turn, recruits dynein-dynactin to NPCs and as such is needed to keep centrosomes closely tethered to the nucleus prior to mitotic entry. When dynein function is suppressed by RNA interference-mediated depletion or antibody microinjection, centrosomes and nuclei are actively pushed apart in late G2 and we show that this is due to the action of
kinesin
-1. Surprisingly, depletion of
BICD2
inhibits both dynein and
kinesin
-1-dependent movements of the nucleus and cytoplasmic NPCs, demonstrating that
BICD2
is needed not only for the dynein function at the nuclear pores but also for the antagonistic activity of
kinesin
-1. Our study demonstrates that the nucleus is subject to opposing activities of dynein and
kinesin
-1 motors and that
BICD2
contributes to nuclear and centrosomal positioning prior to mitotic entry through regulation of both dynein and
kinesin
-1.
...
PMID:Bicaudal D2, dynein, and kinesin-1 associate with nuclear pore complexes and regulate centrosome and nuclear positioning during mitotic entry. 2038 26
