Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.4.4 (
kinesin
)
5,033
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
We developed a new method for imaging the movement of targeted proteins in living cancer cells with photostable and bright quantum dots (QDs). QDs were conjugated with various molecules and proteins, such as phalloidin, anti-tubulin antibody and
kinesin
. These bioconjugated QDs were mixed with a transfection reagent and successfully internalized into living cells. The movements of individual QDs were tracked for long periods of time.
Phalloidin
conjugated QDs bound to actin filaments and showed almost no movement. In contrast, anti-tubulin antibody conjugated QDs bound to microtubules and revealed dynamic movement of microtubules. Kinesin showed an interesting behavior whereby
kinesin
came to be almost paused briefly for a few seconds and then moved once again. This is in direct contrast to the smoothly continuous movement of
kinesin
in an in vitro assay. The maximum velocity of
kinesin
in cells was faster than that in the in vitro assay. These results suggest that intracellular movement of
kinesin
is different from that in the in vitro assay. This newly described method will be a powerful tool for investigating the functions of proteins in living cells.
...
PMID:Intracellular imaging of targeted proteins labeled with quantum dots. 1884 42
