Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.4.4 (kinesin)
 5,033 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Testis brain RNA-binding protein (TB-RBP), the mouse orthologue of the human protein Translin, is a widely expressed and highly conserved protein with proposed functions in chromosomal translocations, mitotic cell division, and mRNA transport, stabilization, and storage. Targeted inactivation of TB-RBP leads to abnormalities in fertility and behavior. A testis-enriched kinesin KIF17b coimmunoprecipitates with TB-RBP in a RNA-protein complex containing specific cAMP-responsive element modulator (CREM)-regulated mRNAs. The specificity of this interaction is confirmed by in vivo RNA-protein crosslinking and transfections of hippocampal neurons. Combining in situ hybridization and immunohistochemistry at the electron microscope level, a temporally sequential dissociation of KIF17b and TB-RBP from specific mRNAs is detected with TB-RBP release coincident with the time of mRNA translation, indicating a separation of the processes of transport and translation. We conclude that KIF17b serves as a molecular motor component of a TB-RBP-mouse ribonucleoprotein complex transporting a group of specific CREM-regulated mRNAs in mammalian male postmeiotic germ cells. Because KIF17b has been reported to control CREM-dependent transcription in male germ cells by regulating the intracellular location of the transcriptional coactivator activator of CREM in testis, this indicates that one kinesin links the processes of transcription and transport of specific mRNAs in mammalian male germ cells.
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PMID:The kinesin KIF17b and RNA-binding protein TB-RBP transport specific cAMP-responsive element modulator-regulated mRNAs in male germ cells. 1467 85

Kinesins are a superfamily of microtubule-based motors that power intracellular traffic and play important roles in many fundamental cellular and developmental processes. Kinesins move on microtubules from their minus to plus end (conventional kinesin) or from plus to minus end (C-terminal kinesins), carrying cargoes to different destinations. A variety of cargoes such as vesicles, proteins, lipid drops, pigments, and the nucleus are moved by kinesins along cytoplasmic microtubules. Multiple mitotic kinesins and microtubule-associated proteins (MAPs) also have direct functions in spindle formation, chromosome segregation, and cytokinesis. Spermatogenesis provides an excellent model system to study the role of kinesin motor proteins during the dramatic cytoskeletal rearrangements that take place during male germ cell development. This chapter describes how to identify the multiple functions of kinesin motors during spermatogenesis by using ultrastructural analysis. Testis perfusion is described in detail, including how to anesthetize animals and how to select seminiferous tubules under transilluminated microscopy. Practical immunocytochemical staining is also described in detail in this chapter, especially methods to enhance staining and avoid contamination.
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PMID:Ultrastructural analysis of kinesin-related motor proteins during spermatogenesis. 1795 15