Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.4.1 (
myosin ATPase
)
1,140
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Carbonic anhydrase (
CA III
) and myoglobin contents from isolated human muscle fibers were quantified using a sensitive time-resolved fluoroimmunoassay. Human psoas muscle specimens were freeze-dried, and single fibers were dissected out and classified into type I, IIA and IIB by
myosin ATPase
staining. Fiber typing was further confirmed by SDS-PAGE.
CA III
and myoglobin were found in all fiber types. Type I fibers contained higher concentrations of
CA III
and myoglobin than type IIA and IIB fibers. The relative concentrations of
CA III
in type IIA and IIB fibers were respectively 24% and 10% of that in type I fibers. The relative concentrations of myoglobin in type IIA and IIB fibers were 60% and 28% of that in type I fibers. Anti-
CA III
immunoblotting results from fiber-specific pooled samples agreed well with quantitative measurements. The results indicate that
CA III
is a more specific marker than myoglobin for type I fibers.
...
PMID:Quantification of carbonic anhydrase III and myoglobin in different fiber types of human psoas muscle. 153 17
Using the indirect immunoperoxidase method, we studied the localization of
carbonic anhydrase
-III (CA-III) in frozen sections of biopsies of human skeletal muscle which had no definite pathology. CA-III was found to be localized in Type-I muscle fibers when compared with serial sections stained with
myosin ATPase
and other reactions. Our finding was in accordance with the biochemical data so far reported. It was though that CA-III could be used as a marker for abnormal Type-I muscle fibers in several neuromuscular diseases.
...
PMID:Carbonic anhydrase-III immunohistochemical localization in human skeletal muscle. 622 2
