Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.4.1 (myosin ATPase)
 1,140 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effects of different thyroid states on some histochemical and biochemical properties of fast-twitch muscle were studied using rat extensor digitorum longus (EDL) muscle. This muscle was found to be much less responsive to thyroidal influence than the slow-twitch soleus muscle. In EDL muscles of hypothyroid rats, fast leads to slow conversions were observed in fibre type composition, myosin ATPase activity and light chain pattern, and in the subunit composition of lactate dehydrogenase, while the only significant slow leads to fast conversion observed in thyrotoxicosis was a decrease in the proportion of slow-oxidative fibres. Denervation of the hypothyroid muscle produced the highest degree of fast leads to slow transformation. These findings support the view that denervation and dysthyreosis alter gene expression in muscle by independent mechanisms.
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PMID:The effects of thyroid status on some properties of rat fast-twitch muscle. 645 58

The objective of this study was to determine the effect of the beta-adrenergic agonist cimaterol (CIM) on fiber characteristics, capillary supply, and metabolic enzyme activities in muscles of young Friesian bulls. Four pairs of monozygotic twins in each of three live weight groups (WG) were used (initial average live weight [LW]: 162, 299, and 407 kg, respectively). Within each pair, one twin was fed .06 mg of CIM.kg LW-1.d-1 for 90 d. The other twin served as control (C). Needle biopsies were obtained from the longissimus (LM) and semitendinosus (ST) muscles at d 82 to 84 of treatment, and muscle fibers were identified as slow-twitch (Type I) or fast-twitch (Type IIA or Type IIB) by the myosin ATPase stain. In LM, the proportion of Type I (C: 24.0%, CIM: 20.4%; P < .07) and Type IIA fibers (C: 24.2%, CIM: 8.6%; P < .001) decreased, whereas the proportion of Type IIB fibers increased (C: 51.7%, CIM: 71.1%; P < .001). Cimaterol increased the cross-sectional area of Type I (P < .02) and Type IIB fibers (P < .001), with no change in Type IIA fibers. Overall, the mean fiber area increased (C: 2,363 microns 2, CIM: 3,934 microns 2; P < .001). The number of capillaries per fiber did not change, but the number of capillaries per square millimeter decreased (P < .001) after CIM treatment. Cimaterol changed metabolic enzyme activities toward lower oxidative capacity of the muscle (lactate dehydrogenase: +22%, hydroxyacyl-CoA dehydrogenase: -33%, and citrate synthetase: -34%; all P < .001) and reduced the glycogen content by 25% (P < .01).(ABSTRACT TRUNCATED AT 250 WORDS)
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PMID:The effect of cimaterol on muscle fiber characteristics, capillary supply, and metabolic potentials of longissimus and semitendinosus muscles from young Friesian bulls. 800 49

Various protocols for the isolation and cultivation of adult rat cardiomyocytes were compared, and the cytoprotective potential of the reversible myosin ATPase inhibitor butanedione monoxime (BDM) was evaluated based on cell yield, cell vitality, lactate dehydrogenase (LDH) and creatine kinase (CK) release, and the mRNA expression of atrial natriuretic peptide (ANP). Overall, a yield of 11.9 x 10(6)cells with >92% cell vitality was obtained when BDM was added to the isolation and cultivation buffers. In contrast, cell vitality ranged from 30% to 70% and cell yield was (4-10) x 10(6) when standard methods for the isolation of cardiomyocytes were used. Butanedione monoxime, at a 15 mM concentration, was cytoprotective during the isolation and cultivation of heart muscle cells, as judged by the morphological appearance (rod shape, lack of bleb formation, and other cytoskeleton defects) and the mRNA expression of the ANP gene. The activities of LDH and CK were also significantly reduced (p < 0.05%) when BDM was added to the isolation and cultivation buffer. The results obtained with BDM warrant further investigation into its cytoprotective potential during ischemia and damage to the cytoskeleton.
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PMID:Butanedione monoxime increases the viability and yield of adult cardiomyocytes in primary cultures. 1221 98

The effect of myosin ATPase inhibitor, 2,3-butanedione monoxime (BDM; used in the range of concentrations 1.25-10.0 mM), on recovery of functions of isolated rat heart subjected to normothermic (37 degrees C) total ischemia for 35 min has been investigated. BDM perfusion was performed at a flow rate of 4 ml/min during 5 min before ischemia (BDM-I) or before 25-min reperfusion (BDM-R). Control hearts were perfused with Krebs solution at the same flow rate. The highest functional recovery of heart and coronary vessels was observed during infusion of 2.5 mM BDM before ischemia. At the end of reperfusion ATP and phosphocreatine (PCr) content in hearts of this group was significantly higher whereas the level of lactate was two times lower than in control; total creatine content (sigmaCr) did not differ from the initial level. Similar but less pronounced changes in the improvement of aerobic metabolism and maintenance of sigmaCr after reperfusion were also observed in the case of infusion of 2.5 mM BDM before reperfusion. They were consistent with reduced recovery of functions of heart and coronary flow compared with these parameters observed in the BDM-I group. 2.5 mM BDM caused almost 2-fold decrease in release of cardiac lactate dehydrogenase into myocardial perfusate in the BDM-I and BDM-R groups (compared with control); this suggests lower damage of cell membranes. These results suggest that improvement of energy supply of postischemic cardiomyocytes may be a key factor determining cardioprotector effectiveness of short-term administration of BDM before ischemia.
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PMID:[The effect of myosin ATPase inhibition on metabolic and functional recovery of isolated rat heart after global ischemia]. 2000 Jan 23


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