Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Two types of magnetic cell sorting assays, termed MRK16-
MACS
and MRK16-
MACS
-FACS, have been established to detect low expression level of
P-glycoprotein
(
P-gp
) using a monoclonal antibody MRK16, which recognizes a cell surface epitope of
P-gp
. With K-562 and U-937 cell lines, which are known to express low levels of
P-gp
and hence routinely used as negative control cell lines in conventional flow cytometry, both assays gave significantly positive reactivities indicating improved specificity and sensitivity of these assays. The findings in the dilution test, where
P-gp
-positive cells were added to
P-gp
-negative cells at various ratios, demonstrated that the MRK16-
MACS
assay is quantitative and capable of detecting small numbers of
P-gp
-positive cells as few as 2.5% of the total cells tested. Furthermore, specific enrichment of
P-gp
-expressing cells in magnetic cell sorting assays was verified by reverse transcription-polymerase chain reaction (RT-PCR) analysis and functional assay for
P-gp
with Rhodamine 123. The availability of such magnetic cell sorting assays may offer an approach to quantitate low level of
P-gp
expression.
...
PMID:Establishment and evaluation of MRK16-magnetic cell sorting assays for detecting low expression of multidrug resistance P-glycoprotein using human leukemia cell lines and peripheral blood cells from healthy donors. 749 Apr 49
Multidrug resistance (MDR) is one of the major obstacles to successful cancer chemotherapy. Since
P-glycoprotein
(
P-gp
) encoded by the MDR-1 gene plays a key role in MDR, many
P-gp
-specific monoclonal antibodies (MAbs) have been generated for characterization and analysis of
P-gp
. Among those antibodies, MRK16 has been widely used not only for elucidation of the mechanisms of
P-gp
-mediated MDR but also for diagnostic and therapeutic studies. Two types of magnetic cell sorting assays, termed MRK16-
MACS
and MRK16-
MACS
-FACS, have been established by us and may offer a useful tool to quantitate low levels of
P-gp
expression. This article describes the characteristics of the antibodies against
P-gp
and discuss the diagnostic implications of the antibodies.
...
PMID:Monoclonal antibodies specific for P-glycoprotein. 920
