Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Some characteristics of doxorubicin-resistant CHO cell line (
RC1
) were studied by means of cell biological methods and SDS-PAGE electrophoresis. The resistance factor was 16.5-fold, and
RC1
revealed cross-resistances to colchicine, actinomycin and harringtonine. By indirect immunofluorescence assay,
P-glycoprotein
was not detected. Compared with CHO, the doxorubicin (Dox) uptake and accumulation of
RC1
decreased, but the membrane fluidity of
RC1
increased. The reduction in drug accumulation was correlated with increase in membrane fluidity. Dox was mainly distributed in the cell nucleus of CHO, but in both cytoplasm and nucleus of
RC1
. This suggested that Dox was transported more slowly in
RC1
cytoplasm than in CHO cytoplasm, resulting in less Dox entrance into the cell nucleus of
RC1
than into that of CHO. We also found that a 30-40 kDa nuclear protein which was expressed normally in CHO disappeared in
RC1
.
...
PMID:[Drug resistance of doxorubicin-resistant CHO cell line]. 751 12
