Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Heterocyclic aromatic amines such as
PHIP
are formed during the heat processing of food.
PHIP
undergoes bacterial metabolism leading to 7-hydroxy-5-methyl-3-phenyl-6,7,8,9-tetrahydropyrido[3',2':4,5]imidazo[1,2-a]pyrimidin-5-ium chloride (PHIP-M1) as main metabolite. We developed an LC-MS method with automated sample preparation by online-solid-phase-extraction for the simultaneous quantification of
PHIP
and its mammalian and bacterial metabolites N-hydroxy-
PHIP
, 4-OH-
PHIP
and
PHIP
-M1 in biological samples. The method was used to investigate the transport of
PHIP
-M1 through a Caco-2 cell monolayer. The experiments show that
PHIP
-M1 rapidly crosses the cell monolayer and that
PHIP
-M1 is a substrate for
P-glycoprotein
and the multiple drug resistance 2 transporter. The intestinal absorption of
PHIP
-M1 is comparable with that of
PHIP
and a moderate to high bioavailability has to be expected. Thus, not only the human metabolites of
PHIP
but also the bacterial metabolite
PHIP
-M1 formed in the gut could contribute to the toxic effects of PhIP.
...
PMID:Development of an online-SPE-LC-MS method for the investigation of the intestinal absorption of 2-amino-1-methyl-6-phenylimidazo[4,5-b]pyridine (PHIP) and its bacterial metabolite PHIP-M1 in a Caco-2 Transwell system. 2505 91
