Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The transepithelial transport of
Cerulenin
across Caco-2 cell monolayers was examined in this study. The permeated amounts of
Cerulenin
were measured by HPLC method to calculate the permeation rate and the apparent permeability coefficient (P(app)). The transport of
Cerulenin
was independent on apical pH and exhibited concentration-dependent and nonsatuable even at 10 mM
Cerulenin
. The permeation rate at 1 mM
Cerulenin
in the apical-to-basolateral direction was 0.151 ng/min/mg of protein and the P(app) was 3.76 x 10(-6) cm/second. The permeation rate of
Cerulenin
was affected by neither metabolic inhibitors nor inhibitors for
P-glycoprotein
, as was the same case in monolayers treated with cytochalasin D. All these data from experiments indicated that transport of
Cerulenin
across Caco-2 cell monolayers was not mediated by ATP-dependent transport systems nor via paracellular pathway, but via passive diffusion without efflux by
P-glycoprotein
.
...
PMID:Transepithelial transport of Cerulenin across Caco-2 cell monolayers. 1964 14
