Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
P-Glycoprotein is an integral membrane protein which mediates the energy-dependent efflux of various antitumor agents from multidrug-resistant cancer cells. Surface plasmon resonance was used for the detection of
P-glycoprotein
after solubilization from drug-resistant and drug-sensitive Chinese hamster ovary cells and for the analysis of its interaction with cyclosporin A, a competitive inhibitor of drug efflux. Detection of
P-glycoprotein
relied on its binding to the monoclonal antibody C219 which was immobilized on a sensor chip. Binding of
Zwittergent 3-14
-solubilized
P-glycoprotein
to the antibody was concentration-dependent and reflected the relative abundance of
P-glycoprotein
in both cell lines. It was abolished when C219 was omitted or replaced by a rabbit anti-mouse IgG antibody and considerably reduced after precipitation of
P-glycoprotein
with wheat germ agglutinin. Preincubation of solubilized proteins with cyclosporin A increased the amount of protein bound to the antibody by approximately 30%. These results indicate that surface plasmon resonance is well suited to the detection of
P-glycoprotein
from biological samples and shows promise as a tool for the study of its interaction with different drugs.
...
PMID:Molecular study of P-glycoprotein in multidrug resistance using surface plasmon resonance. 750 13
