Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The effect of interferon (IFN)-beta and IFN-gamma on
P-glycoprotein
(
P-gp
)-mediated efflux of rhodamin-123(Rho-123), a typical substrate of
P-gp
, was studied in rat hepatocytes in primary culture. After treatment with
IFN-beta
, IFN-gamma, or both for 3 days, steady-state levels of Rho-123, incorporated into the hepatocytes, were measured to evaluate the
P-gp
activity. Whereas
IFN-beta
did not affect the intracellular level of Rho-123, IFN-gamma treatment caused a significant increase of the level, suggesting that IFN-gamma treatment suppresses the expression of
P-gp
or its activity. A combination of the two types of IFN exhibited a similar effect to that of IFN-gamma alone. The effect of IFN-gamma was still observed in the presence of H(2)O(2), which enhances the expression and activity of
P-gp
. Immunoblot analysis using a monoclonal antibody C219 revealed, however, that
P-gp
expression was increased after treatment with IFN-gamma, but only slightly by
IFN-beta
treatment. These results suggest that the enhanced Rho-123 uptake of rat primary hepatocytes induced by IFN-gamma does not result from reduced expression of
P-gp
but, rather, from impaired maturation or dysfunction of the efflux transporter.
...
PMID:Effect of interferons on P-glycoprotein-mediated rhodamine-123 efflux in cultured rat hepatocytes. 1222 38
