Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.3.44 (P-glycoprotein)
 13,344 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The distribution of K(+)-ATPase activity in surface and crypt cells from rabbit distal colon was studied. Separation of surface and crypt cells was validated using the multidrug resistance gene (mdr 1) product, P-glycoprotein, as marker for differentiated surface epithelial cells. Western blot analysis revealed a 6-fold higher expression level of P-glycoprotein in colonic surface cells. K(+)-stimulated ouabain-insensitive ATPase activity was present in surface and in crypt cells. In surface cells, this K(+)-ATPase activity was only partly inhibitable by 10 microM SCH 28080, while in crypt cells K(+)-ATPase activity equalled SCH 28080-sensitive ATPase activity. These results strongly suggest the presence of two distinct K(+)-ATPases in colonic epithelial cells.
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PMID:Two distinct K(+)-ATPase activities in rabbit distal colon. 786 85