Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.44 (
P-glycoprotein
)
13,344
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A small but significant fraction of
pemphigus
patients do not show an adequate response to steroid therapy.
P-glycoprotein
(or P-gp) is a cell membrane efflux pump that expels drugs, including glucocorticoids, from the cytosol to the extracellular medium. An increased expression and/or function of
P-glycoprotein
in lymphoid cells could decrease the intracellular concentration of glucocorticoids, diminishing its therapeutic effects. The aim of this work was to assess the expression and activity of
P-glycoprotein
in the mononuclear cells (MNC) from
pemphigus
patients with good and poor response to steroid therapy. We studied 20 patients with
pemphigus
vulgaris, eight of them classified as poor responders and 12 as good responders to steroid therapy. The expression and activity of
P-glycoprotein
by MNC were quantified by flow cytometry, and
P-glycoprotein
mRNA levels were determined by a semi-quantitative RT-PCR technique. We found that the expression of
P-glycoprotein
at both mRNA and protein levels was similar in
pemphigus
patients with good and poor response to steroid therapy. Similar results were obtained regarding
P-glycoprotein
activity.
P-glycoprotein
does not seem to be involved in the poor response to steroid treatment seen in some
pemphigus
patients. It is important to investigate additional mechanisms that could account for the glucocorticoid resistance seen in some
pemphigus
patients.
...
PMID:Lack of involvement of P-glycoprotein (P-gp) in pemphigus patients with poor response to steroid therapy. 1191 9
