Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.3.14 (ATP synthase)
 7,042 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Epilepsy is a chronic disorder characterized by repeated seizures resulting from abnormal activation of neurons in the brain. Although mutations in genes related to Na(+), K(+), Ca(2+) channels have been defined, few studies show intracellular protein changes. We have used proteomics to investigate the expression of soluble proteins in a genetic rat model of absence epilepsy "Genetic Absence Epilepsy Rats from Strasbourg (GAERS)". The advantage of this technique is its high throughput quantitative and qualitative detection of all proteins with their post-translational modifications at a given time. The parietal cortex and thalamus, which are the regions responsible for the generation of absence seizures, and the hippocampus, which is not involved in this activity, were dissected from GAERS and from non-epileptic control rat brains. Proteins from each tissue sample were isolated and separated by two-dimensional gel electrophoresis. Spots that showed significantly different levels of expression between controls and GAERS were identified by nano LC-ESI-MS/MS. Identified proteins were: ATP synthase subunit delta and the 14-3-3 zeta isoform in parietal cortex; myelin basic protein and macrophage migration inhibitory factor in thalamus; and macrophage migration inhibitory factor and 0-beta 2 globulin in hippocampus. All protein expressions were up-regulated in GAERS except 0-beta globulin. These soluble proteins are related to energy generation, signal transduction, inflammatory processes and membrane conductance. These results indicate that not only membrane proteins but also cytoplasmic proteins may take place in the pathophysiology and can be therapeutic targets in absence epilepsy.
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PMID:Changes in intracellular protein expression in cortex, thalamus and hippocampus in a genetic rat model of absence epilepsy. 2131 Feb 18

Exercise has beneficial effects on brain function, including the promotion of plasticity and the enhancement of learning and memory performance. To further explore the molecular changes in the protein expression levels in the cerebral cortex of physically trained rats, male Wistar rats 8 months old were selected and subjected to swimming training in a rectangular glass tank for 30 min/day, 6 days/week and 3% of load for 4 weeks. Two dimensional gel electrophoresis followed by mass spectrometrical identification assigning spots to proteins and determination of coomassie-densities of the protein spots were carried out. The up-regulated spots were found to be statistically significant with a p-value of <0.05. Proteins related to energy metabolism like GAPDH and ATP synthase and synaptic plasticity related proteins like actin, tubulin and 14-3-3 zeta/delta were up-regulated in the cerebral cortex of swim trained rats. Thus, our results suggest that exercise elicits a differential protein expression pattern with significant changes in proteins relevant to cortical function and these proteins may be incorporated with neuronal recovery in terms of neurite formation and remodeling of synaptic connections.
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PMID:Differential expression of the cerebral cortex proteome in physically trained adult rats. 2476 25