Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.3.14 (ATP synthase)
 7,042 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Cholate-solubilized F0 complexes of the ATP synthase (F0F1) from Escherichia coli were studied by application of conventional transmission electron microscopy and electron spectroscopic imaging (ESI) of negatively stained samples. Using the ESI mode, the structural organization of the F0 complex (diameter of 7.5 +/- 0.5 nm) could be observed in more detail and defined projections could be distinguished. Projection A appears as a deltoid-like structure with bilateral symmetry. Projection B has an overall trapezoidal shape with some similarity in shape to the letter W. Applying the ESI mode to the ac complex dissolved in cholate-containing buffer, an elongated structure consisting of two intensity maxima could be observed. Simulations with models of the F0 and the ac complex revealed that the projections observed can be obtained by tilting and rotating a model in which subunit a and the two copies of subunit b are located outside the subunit c oligomer. This view of structural organization was supported by results obtained with F0 complexes decorated with monoclonal antibodies against subunits a, b or c.
 ...
PMID:The F0 complex of the Escherichia coli ATP synthase. Investigation by electron spectroscopic imaging and immunoelectron microscopy. 760 Nov 25

The synthesis of taurocholic and glycocholic acids was studied in the whole homogenate and in subcellular fractions of livers from 18 patients operated on for cholestasis and compared with a control material with clinically normal liver function. A considerable decrease of synthesis of cholic acid conjugates was found in icteric homogenates, while no significant alteration of the synthesis was found in subcellular fractions containing the bile acid activating and transferring enzyme. The synthesis of cholic acid conjugates in icteric homogenates showed an inverse significant correlation to serum bilirubin values. Cholic acid conjugates added in vitro inhibited the synthesis of conjugates in homogenates and subcellular fractions. Bilirubin added in vitro brought about a significant decrease in synthesis of conjugates in homogenates but no changes of synthesis in the subcellular fractions mentioned. Soluble fraction as well as isolated mitochondria obtained from icteric human liver inhibited the synthesis of conjugates in microsome fraction and in combined microsome fraction and L-fraction. Oligomycin significantly increased the synthesis of conjugates in icteric homogenates but did not influence the synthesis in the mentioned subcellular fractions from icteric livers, or in homogenates from livers with clinically normal function. In no case, however, did oligomycin raise the synthesis of bile acid conjugates in icteric homogenate to that of controls. It is suggested that the bile acid conjugating enzyme system is intact in cases of cholestasis of short duration (<14 days) and that the decreased synthesis of conjugates in icteric homogenates might be the result of a product inhibition of the conjugation reaction by retained bile acid conjugates and of stimulated mitochondrial ATPase activity caused by retention of bile components.
 ...
PMID:The synthesis of taurocholic and glycocholic acids in human liver homogenates and subcellular fractions in obstructive jaundice. 2018 67