Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.14 (
ATP synthase
)
7,042
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Plant height (PH) is one of the most important agronomic traits of rice, as it directly affects the yield potential and lodging resistance. Here, semi-dwarf mutant lines were developed through CRISPR/Cas9-based editing of OsGA20ox2 in an indica rice cultivar. Total 24 independent lines were obtained in T
0
generation with the mean mutation rate of 73.5% including biallelic (29.16%), homozygous (47.91%) and heterozygous (16.66%) mutations, and 16 T-DNA-free lines (50%) were obtained in T
1
generation without off-target effect in four most likely sites. Mutations resulted in a changed amino acid sequence of mutant plants and reduced gibberellins (GA) level and PH (22.2%), flag leaf length (FLL) and increased yield per plant (YPP) (6.0%), while there was no effect on other agronomic traits. Mutants restored their PH to normal by exogenous GA
3
treatment. The expression of the
OsGA20ox2
gene was significantly suppressed in mutant plants, while the expression level was not affected for other GA biosynthesis (OsGA2ox3 and OsGA3ox2) and signaling (D1, GIDI and SLR1) genes. The mutant lines showed decreased cell length and width, abnormal cell elongation, while increased cell numbers in the second internode sections at mature stage. Total 30 protein spots were exercised, and 24 proteins were identified, and results showed that
OsGA20ox2
editing altered protein expression. Five proteins including, glyceraldehyde-3-phosphate dehydrogenase, putative
ATP synthase
, fructose-bisphosphate aldolase 1,
S
-adenosyl
methionine
synthetase 1 and gibberellin 20 oxidase 2, were downregulated in dwarf mutant lines which may affect the plant growth. Collectively, our results provide the insights into the role of
OsGA20ox2
in PH and confirmed that CRISPR-Cas9 is a powerful tool to understand the gene functions.
...
PMID:Generation of semi-dwarf rice (
Oryza sativa
L.) lines by CRISPR/Cas9-directed mutagenesis of
OsGA20ox2
and proteomic analysis of unveiled changes caused by mutations. 3165 25
Despite recent progress in hepatitis treatment, there have been no significant advances in the development of liver cancer vaccines in recent years. In this study, we investigated the regulatory effect and potential mechanism of hepatocyte growth factor receptor (
MET
, also known as HGFR) on tumor vaccinations for liver cancer in mice. Herein, we demonstrate that
MET
expression is significantly associated with the immunogenicity of liver cancer in mice and humans, and that
MET
depletion dramatically enhances the protective efficacy of chemotherapy-based anti-liver cancer vaccination. Mechanistically,
MET
repressed liver cancer immunogenicity independent of the traditional PI3K-AKT cascade, and
MET
interacted with vacuolar
ATP synthase
(V-ATPase) and mediated the activation of mammalian target of rapamycin (MTOR), thus suppressing liver cancer immunogenicity. The efficacy of chemotherapy-based liver cancer vaccination was markedly enhanced by targeting the
MET
-V-ATPase-MTOR axis, highlighting a translational strategy for identifying
MET
-associated drug candidates for cancer prevention.
...
PMID:The AKT-independent MET-V-ATPase-MTOR axis suppresses liver cancer vaccination. 3276 35
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