Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.3.14 (ATP synthase)
 7,042 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Isolated myocytes from ventricles are quiescent in the presence of 0.9 mM calcium. However, it is possible to induce beating by adding 0.5 mM BaCl2 to the media or to induce a contracture by elevating the external concentration of potassium (72.5 mM K). During the viable stage of contracture, which is up to 1 h, the sarcomere spacing is 1.7 +/- 0.1 micron and no leakage of intracellular components is observed. The metabolic properties of the cells in quiescent, beating and contracted states were compared. The O2 consumption (natom per mg cell protein per min) increased from 10-11 in quiescent cells to 60-66 in beating cells and 90-99 in contractured cells. In contrast no significant difference was found in the metabolite levels in the three cellular states: (nmol per mg cell protein +/- S.E.M.) ATP, 20.9 +/- 1.5; CrP, 22.3 +/- 2.2; ADP, 6.03 +/- 0.67; Cr, 10.8 +/- 1. It is proposed that the combined action of myosine ATPase, ATP synthase and cytosolic and mitochondrial creatine kinases serves to buffer the metabolite levels during periods of enhanced oxygen consumption.
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PMID:High-energy phosphates in quiescent, beating and contracted cardiac cells. 326 May 16