Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.3.14 (ATP synthase)
 7,042 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The structural organization of an expressed bovine gene (ATPA1) that encodes an isoform of the alpha-subunit of the mitochondrial F0F1 ATP synthase was determined. The gene extends over 10 kilobase-pairs and is divided into 12 exons. The first exon encodes the 5' untranslated region and approximately one-half of the presequence that targets this protein to the mitochondrion. The remainder of the presequence, together with three amino acids of the mature protein, are encoded by exon 2. Primer extension and nuclease protection analyses revealed multiple sites of transcription initiation. The 5' flanking region of the ATPA1 gene can drive the transcription of a reporter gene in an orientation-dependent manner. This promoter region contains several sequence elements which might play an important role in regulating the expression of this gene, including possible TATA and CCAAT boxes, putative Sp1-binding sites, and sequences resembling AP-1, AP-2, AP-4 and cAMP-responsive elements. The ATPA1 gene also contains sequences homologous to several motifs that are shared among some nuclear genes encoding mitochondrial proteins. These include Mt1, Mt3, Mt4, a respiratory enhancer, and NRF-2 sites. Tissue-specific differences in the ATPA1 mRNA levels were observed with high levels found in skeletal muscle and heart, and lower levels in other tissues.
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PMID:Structural organization of a nuclear gene for the alpha-subunit of the bovine mitochondrial ATP synthase complex. 142 Mar 6

The structural organization of the entire human nuclear encoded gene for mitochondrial cytochrome c1 was determined by analyzing a clone obtained from an EMBL3 genomic DNA library. The gene spans 2.4-kilobase pairs and contains seven exons interrupted by six introns of relatively small sizes. All intron/exon splice junctions follow the GT/AG rule. The 5'-flanking region of the gene lacks typical transcriptional regulatory sequence elements such as TATA and CAAT boxes but contains seven putative GC boxes (Sp1 binding sites) and several sequences that resemble another type of the Sp1 responsive element, the enhancer core consensus sequence, the AP-1 responsive element, and the cAMP- and phorbol ester-inducible element. The region also contains a 15-nucleotide sequence highly homologous to the AP-4 consensus sequence and to those in the 5'-flanking regions of the genes for two enzymes associated with respiratory function, the beta subunit of human ATP synthase and chicken 5-aminolevulinate synthase. The presequence, which is essential for the transport of the cytochrome c1 precursor into mitochondria, is encoded in both the first and second exons, and the nucleotide sequence corresponding to the presequence is separated by the first intron. This is the first example of a leader sequence coding for a presequence clearly separated into two parts by an intron.
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PMID:Structural organization of the human mitochondrial cytochrome c1 gene. 253 65