Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.3.14 (ATP synthase)
 7,042 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Having used 2-mercaptopropionylglycine in successful treatment of chronic hepatitis the mode of action of the reagent was further studied in rat liver mitochondrial membrane. Gradient polyacrylamide gel electrophoresis was employed for this purpose. It was found that after incubation of the mitochondria with ADP and 2-mercaptopropionylglycine and isolation of mitochondrial membranes the amount of low-molecular weight polypeptides was significantly increased. These polypeptides belong to the molecular weight ranges of the epsilon-subunits of the mitochondrial ATPase and of the oligomycin binding site. A working hypothesis is formed that the drug may improve anchoring the ATPase molecule at the membrane site. Such mechanism may elucidate previous biochemical findings, and, in particular, underlines the structure-preserving effect of 2-mercaptopropionylglycine in mitochondria.
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PMID:Increase of low-molecular weight polypeptides of rat liver mitochondrial membrane by 2-mercaptopropionylglycine. 719 Apr 5

Chronic infections with hepatitis B (HBV) and hepatitis C (HCV) viruses are major risk factors for hepatocellular carcinoma (HCC). We have utilized a proteomic approach to determine whether a distinct repertoire of autoantibodies can be identified in HCC. Sera from 37 patients with HCC and 31 subjects chronically infected with HBV or HCV without HCC were investigated. Sera from 116 patients with other cancers, three patients with systemic lupus erythematosus, and 24 healthy subjects were utilized as controls. We report the identification of eight proteins, for each of which autoantibodies were detected in sera from more than 10% of patients with HCC but not in sera from healthy individuals (p < 0.05). Autoantibodies to four of these proteins were detected at a comparable frequency in sera from patients with chronic hepatitis. The other four proteins, which consisted of calreticulin isoforms, cytokeratin 8, nucleoside diphosphate kinase A, and F(1)-ATP synthase beta-subunit, induced autoantibodies among patients with HCC, independently of their HBV/HCV status. Calreticulin, and a novel truncated form of calreticulin (Crt32) we have identified, most commonly elicited autoantibodies among patients with HCC (27%). We conclude that a distinct repertoire of autoantibodies is associated with HCC that may have utility in early diagnosis of HCC among high risk subjects with chronic hepatitis.
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PMID:A distinct repertoire of autoantibodies in hepatocellular carcinoma identified by proteomic analysis. 1209 19