Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.3.1 (Mg2+-ATPase)
 1,484 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The ATP-dependent Ca2+ uptake by and the Ca2+-Mg2+-activated ATPase activity in the subcellular fractions from the eggshell gland of two varieties of ducks, Indian Runner ducks (IRD) and the cross-breed of Swedish and Rouen ducks (SRD), showed functional changes during eggshell formation in relation to the values in the resting state. DDE inhibited the Ca2+ uptake (Ca) but increased the Ca2+-Mg2+-ATPase activity (P) of fractions from active but not of those from resting glands. DDE reduced the Ca/P ratio of several fractions from active glands. DDE inhibited the functional increase in Ca2+-Mg2+-ATPase in the IRD homogenate, however. The effect of DDE on the eggshell index of mature eggshell was greater in IRD than in SRD, the reason for this being that the effect was exerted during a shorter time period in the latter variety.
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PMID:Relation between Ca metabolism and ATPase activities in the subcellular fractions from duck eggshell gland mucosa after DDE administration during different stages of eggshell formation. 286 48

Thinning of the egg shell is produced by p-p'-DDT and DDE in several species of birds. A study was made of the effect of DDE administered in vitro and in vivo on the Ca2+ binding and Ca2+-Mg2+-activated ATPase of a homogenate of the egg shell gland of ducks (Anas platyrhynchos var.). The concentration of Ca2+ was 1 X 10(-4) M and that of MgATP 1 X 10(-3) M. In vitro, DDE in concentrations of 2-16 micrograms/ml of incubation medium inhibited the Ca2+-Mg2+-activated ATPase in a concentration-dependent manner, whereas Mg2+-activated ATPase was not affected by these concentrations. The Ca2+ binding by the homogenate was reduced by DDE in the same concentrations. The sodium azide sensitive Ca2+ binding was most sensitive. In vivo, DDE administered in a concentration of 40 mg/kg dry weight of the food for 45 days reduced the egg shell index by 18% in comparison to controls. After 45 days of treatment the DDE concentrations in the egg shell gland mucosa was 1.20 +/- 0.16 micrograms/g of wet weight, while no DDE was detected in the controls. The Ca2+-Mg2+-activated ATPase was reduced by 32%, whereas the Mg2+-ATPase was not changed. The Ca2+ binding by the homogenate was reduced by 29%, the sodium azide sensitive part being most vulnerable, DDE increased the total Ca content of the egg shell gland mucosa by 44%. Since Ca is transported against a concentration gradient between blood plasma, and the lumen of the shell gland, it is suggested that DDE, by inhibiting the Ca2+-Mg2+-activated ATPase, decreased the Ca translocation over the egg shell gland mucosa.
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PMID:Effect of p-p'-DDE administered in vivo and in vitro on Ca2+ binding and Ca2+-Mg2+-ATPase activity in egg shell gland mucose of ducks. 612 33