Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
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Drug
Enzyme
Compound
Query: EC:3.6.3.1 (
Mg2+-ATPase
)
1,484
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A protein which supported MgATP-dependent movement of latex beads from the minus to the plus end of microtubules and which had microtubule-activated
Mg2+-ATPase
was purified from Acanthamoeba castellanii. At concentrations as low as 0.6 micrograms ml-1, the translocator supported movement of beads at a rate of 3 to 4 micron s-1. The
translocator protein
had a Ca2+-ATPase activity of 1.7 mumol min-1 mg-1 and a
Mg2+-ATPase
activity of about 0.03 mumol min-1 mg-1 in the absence of microtubules. The
Mg2+-ATPase
in the presence of microtubules had a Vmax of 3.4 mumol min-1 mg-1; half-maximal
Mg2+-ATPase
activity required only 0.45 microM microtubules (concentration of dimer subunits). The highly purified native protein had a Stokes radius of 8.5 nm, and three polypeptides of Mr 134,000, 139,000, and 147,000 were associated with the fractions that had maximum translocator and ATPase activities.
...
PMID:Extensive purification from Acanthamoeba castellanii of a microtubule-dependent translocator with microtubule-activated Mg2+-ATPase activity. 296 Jun 74
