Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.3.1 (
Mg2+-ATPase
)
1,484
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Cell polarity is a fundamental feature of all multicellular organisms.
PIN
auxin transporters are important cell polarity markers that play crucial roles in a plethora of developmental processes in plants. Here, to identify components involved in cell polarity establishment and maintenance in plants, we performed a forward genetic screening of
PIN2
:
PIN1
-
HA
;
pin2
Arabidopsis (
Arabidopsis thaliana
) plants, which ectopically express predominantly basally localized PIN1 in root epidermal cells, leading to agravitropic root growth. We identified the
regulator of
PIN
polarity 12
(
repp12
) mutation, which restored gravitropic root growth and caused a switch in PIN1-HA polarity from the basal to apical side of root epidermal cells. Next Generation Sequencing and complementation experiments established the causative mutation of
repp12
as a single amino acid exchange in Aminophospholipid ATPase3 (ALA3), a phospholipid
flippase
predicted to function in vesicle formation.
repp12
and
ala3
T-DNA mutants show defects in many auxin-regulated processes, asymmetric auxin distribution, and
PIN
trafficking. Analysis of quintuple and sextuple mutants confirmed the crucial roles of ALA proteins in regulating plant development as well as
PIN
trafficking and polarity. Genetic and physical interaction studies revealed that ALA3 functions together with the ADP ribosylation factor GTPase exchange factors GNOM and BIG3 in regulating
PIN
polarity, trafficking, and auxin-mediated development.
...
PMID:Arabidopsis Flippases Cooperate with ARF GTPase Exchange Factors to Regulate the Trafficking and Polarity of PIN Auxin Transporters. 3221 33
