Gene/Protein Disease Symptom Drug Enzyme Compound
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Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Influence of fibrinogen degradation products (FDP) on the ATPase activity in the rat heart. Acta Physiol. Pol., 1978, 29 (2): 185--187. The influence of dialysable fibrinogen degradation products (FDP) on the ATPase activity was studied. It was found that FDP augment the Mg(2+)-- dependent ATPase and slightly decrease the (Na+--K+) dependent ATPase in the rat heart. It is concluded that biological activity of examined peptides depend on other mechanisms than the direct effect on ATPase in the heart.
Acta Physiol Pol
PMID:Influence of fibrinogen degradation products (FDP) on the ATPase activity in the rat heart. 14 31

Alkaline inorganic pyrophosphatase and Mg-ATPase are localized within the mitoplast of maize seeding mitochondria. NaF inhibited the PPase activity, whereas oligomycin and dicyclohexylcarbodiimide inhibited the Mg-ATPase activity. The mitoplast preparation synthesized PPi from Pi under conditions excluding hydrolysis of endogenous ATP. PPi synthesis was inhibited by ADP, antimycin A, NaCN and 2,4- dinitrophenol but not by oligomycin. It is suggested that PPi synthesis in the maize seedling mitochondria proceeds at the expense of the energy of electron transport chain and is independent of the ATP synthesis.
Acta Biochim Pol 1978
PMID:Submitochondrial localization and function of alkaline inorganic pyrophosphatase in maize seedlings. 15 79

1. Heavy microsomal fraction (HM) of rabbit skeletal muscle obtained by differential centrifugation between 8 000-30 000 g and consisting of sarcoplasmic reticulum (SR) vesicles contains variable amounts of glycogen and reveals some activity of phosphorylase b. The monomer of this enzyme of mol. wt. about 100 000 co-migrates in SDS-polyacrylamide gel electrophoresis with the main SR protein--Ca2+,Mg2+--dependent ATPase. 2. The highest specific activity of phosphorylase and the highest content of glycogen is present in the light microsomal (LM) fraction (30 000-100 000 g). 3. Contrary to the ATPase, phosphorylase b is released from the microsomal fraction by treatment with EDTA and is resistant to trypsin. 4. Both HM and LM fractions can be further fractionated on continuous sucrose density gradient at high speed. Main fraction of HM consists of highly purified SR vesicles. The second, small fraction of HM is identical with the main fraction of LM and consists of two populations: vesicles of structure and properties different from those of SR vesicles, and the particles of a complex of glycogen with some glycolytic enzymes.
Acta Biochim Pol 1977
PMID:Sarcoplasmic reticulum vesicles and glycogen-protein particles in microsomal fraction of skeletal muscle. 87 37

The enzymatic examinations were carried out to determine, as early as possible, the functional changes in cells of fetal and maternal part of placenta, as well as pointing out those elements which were the most susceptible to cadmium toxic action. The experiments were carried out on 80 pregnant female rats which were divided into three experimental groups and a control one. The experimental animals were daily administered, intragastrically, aqueous solution of cadmium chloride, between the 7th and 19th day of pregnancy, in doses depending on experimental groups: 2, 10 and 20 mg of CdCl2 per kilogram of body weight, respectively, the animals were killed on the 21st day of pregnancy. Placenta sections were determined for activity of adenosinetriphosphatase stimulated by Mg++ ions (ATP-ase Mg++) E.C.3.6.1.3., and acid phosphatase (AcP) E.C.3.1.3.2. When administered to pregnant females, cadmium chloride was proved to cause a considerable impairment of active transport through biological membranes in placenta, which is indicated by a decrease of reactivity to Mg++ ions stimulated ATP-ase; and an increase in intracellular catabolic processes, which in turn is shown by an increase in reactivity to acid phosphatase. The fetal part of the placenta proves to be more susceptible to the lesion causing activity of cadmium than the maternal part of this organ.
Ginekol Pol 1992 Jun
PMID:[Examination of activity of certain phosphatases in the placenta of female rats exposed to cadmium]. 130 27

In the lymphocytes infected in vitro with BLV (bovine leukemia virus) the contents of Ca2+ and Mg2+ were determined using roentgen microanalyser JXA-5 A Joel-form (Japan). In the smears prepared from these cells the activity of enzyme markers of cell membranes i.e. alkaline phosphatase (AP - EC 3.1.3.1), 5'-nucleotidase (5'-NT - EC 3.1.3.5) and adenosine-triphosphatases - Ca2+ and Mg2+ dependent (ATP-ase - EC 3.6.1.3) was determined. The decrease in AP and ATP-assess activity and increase in 5'-NT in the membranes of leukemic lymphocytes were observed. During these changes the increase in Ca2+ and decrease in Mg2+ ions occurred. These processes lead to clear disturbances in the metabolism of cells transformed by the neoplasm. The effect of this phenomenon is probably the opening of calcium canals with the following cytoplasmatic hypercalcemia. It's very destructive for the change in permeability of the membrane of lymphocytes.
Pol Arch Weter 1991
PMID:[The content of Ca2+ and Mg2+ ions and membrane enzyme activity (AP, 5'-NT, ATPases) in the lymphocytes infected in vitro with bovine leukemia virus]. 166 9

In the presence of NADPH cytochrome P-450-dependent monooxygenases oxidize arachidonic acid giving rise to four epoxyeicosatrienoic acids (EETs) which are hydrolyzed enzymatically to dihydroxyeicosatrienoic acids (DHETs). EETs generate vasodilators. Allylic oxidation forms hydroxyeicosatetraenoic acids, of which 12(R)HETE is an inhibitor of Na(+)-K(+)-ATPase and renin release. Finally, omega and omega-1 hydroxylation of arachidonic acid generates 20- and 19-HETEs which are involved in the development of hypertension in SHR rats.
Pol J Pharmacol Pharm
PMID:Cytochrome P-450 metabolites of arachidonic acid: implications for blood pressure regulation. 212 86

It was found, using circular dichroism spectroscopy, that CaM, in the presence of Ca2+, decreases the alpha-helix content of (Ca2(+)-Mg2+)ATPase of porcine erythrocytes from 66% to 55%. In the absence of Ca2+ the enzyme showed 46% of alpha-helix. Moreover, quenching of the ATPase intrinsic fluorescence by acrylamide indicated that, depending on the enzyme conformational status, the accessibility of its tryptophan residues is influenced by direct interaction with CaM at micromolar Ca2+ concentration. This was also confirmed by the observation that fluorescence energy transfer occurred from tryptophan residues of (Ca2(+)-Mg2+)ATPase to dansylated CaM. The presented results may indicate that binding of CaM gives rise to a novel conformational state of the enzyme, distinct from E1 and E2 forms of the Ca2+ pump.
Acta Biochim Pol 1990
PMID:Circular dichroism and fluorescence studies on interaction of calmodulin (CaM) with purified (Ca2(+)-Mg2+)ATPase of erythrocyte ghosts. 215 Sep 4

In the present study, the effects of low- and non-toxic high doses of acetylsalicylic acid (ASA) on liver plasma membrane Ca2+ ATPase activity and also on some membrane lipid components were investigated in rats. ASA was administered to rats by means of a gastric tube at doses of 50 mg/kg and 200 mg/kg, daily, for 30 days. Chronic oral administration of high dose of ASA resulted in significant increases in liver plasma membrane cholesterol and phospholipid levels, whereas the membrane lipids appeared not to be affected by low dose of ASA. Liver plasma membrane Ca2+ ATPase activity was found to be significantly inhibited following high dose of ASA treatment. Low dose of ASA administration caused a slight, but non-significant decrease in enzyme activity. It is concluded that the inhibition of Ca2+ ATPase activity produced by high dose of ASA treatment may be expected to cause Ca2+ accumulation in liver cells, thereby leading to the cell injury.
Pol J Pharmacol Pharm
PMID:Effect of acetylsalicylic acid on liver plasma membrane Ca2+ ATPase activity. 215 33

RNA-dependent ATPase activity of Rho + and two mutant proteins Rho15 and Rho301 was studied. It was shown that monomeric Rho forms oligomers in the presence of ATP. This ATP-induced structural change of Rho allows protection of the protein from heat inactivation. Poly(C), which highly activates Rho ATPase, was found to potentiate heat inactivation of Rho301, but no Rho + and Rho15, only under optimal conditions of ATP hydrolysis. It was also shown that Rho301 is defective in interaction with RNA. The molecular model postulating that Rho-catalysed ATP hydrolysis with free RNA involves the cyclic process of protein dissociation and reassembly is postulated.
Acta Microbiol Pol 1986
PMID:Free RNA-dependent ATPase activity of transcription termination factor Rho: a model of cyclic dissociation and reassembly of Rho protein. 242 24

Supplementation of the growth medium with erosterol, cholesterol and lanosterol enriched the Candida kefyr cells, presumably cell membranes with sterols. Sterol enriched C. kefyr cells showed a decrease in percentage of PHA and Con-A mediated agglutination. Sterol supplementation also increased the sterol: phospholipid ratio and in such cells unsaturated fatty acids predominated over saturated ones. The overall effect of these changes resulted in rigidifying the cell membranes as indicated by shift of break in Arrhenius plots of Mg2+ ATPase. This showed that lectin mediated agglutination of yeast cells may be affected by its membrane fluidity.
Acta Microbiol Pol 1988
PMID:Lectin mediated agglutination of Candida kefyr cells and their spheroplasts grown in sterol enriched growth medium and its correlation with lipid composition. 246 28


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