Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

The effect of peroral administration of "Cynkotox" (diethyl-dithio-carbamate, zinc salt) on the histoenzymatic activity of the brain was examined. 1.0 g of Cynkotox was given daily to adult Wistar rats over a period of 10 days (the per os DL50 for rats is 5200 mg/kg of body weight). The results have shown that chronic ingestion of large doses of Cynkotox may cause degenerative changes of neurons in various brain regions as well as alterations in the activity of various enzymes. The cerebral activities TPPase and of acP were evidently increased, whereas those of AChE, BuTJ and ATPase were markedly reduced. In some regions of the experimental rat brains. NsE activity was elevated in other ones some loss of NsE activity was noticed.
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PMID:The activity of some hydrolytic enzymes in the brain after administration of "Cynkotox". 611 90

The effect of ingestion of phenylmercury acetate (PhMA), a fungicide of wide agricultural application, on the histoenzymatic pattern of the thalamic nuclei was investigated. It has been shown that in rats administered via a gastric tube with 8 mg of PhMA for 10 consecutive days, a considerable drop of AChE, BuTJ, acP and ATPase activities in many thalamic nuclei was found. Beside the inhibition of many enzymes, TPPase and NsE activities were increased. The histoenzymatic response of the individual thalamic nuclei to PhMA intoxication was highly differentiated. A detailed list of results obtained are presented in tables I and II.
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PMID:Histoenzymatic changes in the thalamic nuclei of rats intoxicated by phenyl-mercury acetate (PhMA). 612 Jan 92

Organic Mercury compounds are known for their neurotoxicity. From our previous studies it appeared that a variable degree of damage is brought about in the individual structures of the CNS by ingestion of organic mercurials. A detailed study was undertaken on the histotopography of pathological changes occurring in the thalamus - the major integrator of various cerebral functions - following intragastric administration of EMTS, a fungicide widely applied in agriculture. Rats served as the experimental animals. They were treated intragastrically with a daily dose of 15 mg of EMTS over a period of 10 days ( the per os DL50 for rats is 100 mg/kg of body weight). The results of our study have revealed that chronic ingestion of large doses of EMTS brings about a considerable inhibition of the activities of AChE, BuTJ, acP and ATPase in many thalamic nuclei with a concomitant elevation of TPPase and NsE activities. There was considerable variation as to the degree of histoenzymatic changes in the individual thalamic nuclei. The accompanying glial reaction was evaluated aw well. An interpretation of the findings made in this study has been undertaken.
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PMID:Morphology and histochemistry of the thalamus in the course of intoxication by ethyl-mercury-p-toluenesulphanilide (EMTS). 612 14

1. Crude synaptosomal fractions obtained from four areas of rat brain were studied; cerebral cortex, hippocampus, midbrain, thalamus with hypothalamus, using the Cotman & Matthews method (1971) Biochim, biophys. Acta 249, 350-394. 2. The purity of synaptosomal fractions was controlled by electron microscopy, and by determination of some marker enzymes such as: LDH, MAO, AChE and cytochrome-c oxidase. 3. Synaptosomes were disrupted by hypoosmotical shock. 4. Crude synaptosomal membrane preparations indicated on increased Ca2+ Mg2+ -ATPase activity in comparison to the activity of this enzyme in synaptosomal fractions. 5. The incubation of crude synaptosomal membranes with cAMP and theophylline caused the subsequent increase of Ca2+ Mg2+ -ATPase activity, but mainly, in hippocampal region.
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PMID:Ca2+ Mg2+ -ATPase activity of synaptosome fraction and synaptosomal membranes from different areas of rat brain. 613 9

Adult Wistar rats of either sex were treated intraperitoneally on alternate days with 0.0015 mg of Vincristin over a period of 24 days, and their brains examined with respect to histoenzymatic activity. It has been found that the adopted treatment resulted in a drop of the cerebral AChE, alkP, acP and ATPase activities. The activity of NsE was depressed in some regions of the brain, whereas in others an enhancement of NsE activity was found. TPPase activity appeared to increase following treatment with Vincristin. The observed differential reaction of NsE to Vincristin treatment could be indicative of an existence of multiple isoenzymes of carbocylesterases in the brain. The moderate intensity of changes in the enzymatic activity of the experimental brains evoked by the treatment of rats with Vincristin makes it feasible to assume that after cessation of the treatment the original enzymatic activity of the brain will be restored.
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PMID:The effect of vincristine on enzymatic activity in the brain. 614 86

It is shown that when one of the olfactory bulbs (OB) of the rat is deprived of olfactory stimulation from birth, as compared to the normal OB on the opposite side, significant and permanent reductions in its growth and in several biochemical parameters take place as follows: weight gain, 25%; total DNA, 20--30%; total RNA, 30%; total protein, 30%; total Na-K-ATPase activity, 50%; total AChE activity, 20%. The concentrations of DNA, RNA and protein and the specific activity of AChE were not significantly affected but the specific activity of Na-K-ATPase was significantly reduced. In general, these interbulbar differences were seen in every experimental animal and were not due to hypertrophy or hyperplasia of the normal OB. These results suggest that olfactory stimulation during the early postnatal period has a significant influence on cell proliferation and cell growth as well as on the proliferation of neuronal membranes and synapses in the developing OB. It appears that the effects of olfactory deprivation are exerted during the first few weeks after birth when the bulb shows its most rapid period of growth and development, since (with the exception of DNA) the magnitude of the interbulbar difference did not increase appreciably by prolongation of olfactory deprivation beyond the early period. Thus in the rat the existence of a critical period encompassing the second and third postnatal weeks is suggested during which the developing OB appears to be especially vulnerable to the absence of olfactory stimulation.
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PMID:Lasting effects of early olfactory deprivation on the growth, DNA, RNA and protein content, and Na-K-ATPase and AchE activity of the rat olfactory bulb. 616 39

The present paper deals with the correlative histochemical and biochemical studies of the epididymis following the treatments of alpha-chlorohydrin. This drug was administered in chronic low dose (15 mg/kg body weight/day) for 20 and 30 days and a single high dose (90 mg/kg body weight). Histochemical alterations of ATPase, SDH and AChE were studied in various components of epididymal epithelium and the total enzyme content was measured by biochemical parameters. The study shows progressive decrease of the enzymes in the interstitium and the epithelium of both the caput and cauda epididymes with increasing dose and duration, except for the high dose effect of alpha-chlorohydrin on AChE. Since alpha-chlorohydrin decreases the androgen dependent enzymes (ATPase, SDH, AChE), there is a possibility that the drug may be antiandrogenic in nature. In such case the action of these drugs may not be directly on the spermatozoa, as proposed by earlier workers, but is mediated by changing the physiology of the epididymis, affecting the milieu in which the spermatozoa mature.
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PMID:Correlative histochemical and biochemical studies on the adenosine triphosphatase, succinic dehydrogenase and acetylcholinesterase in the epididymis of mice after alpha-chlorohydrin treatment. 623 98

The activity of Na+, K+ATPase (EC 3.6.1.3) and acetylcholinesterase (EC 3.1.1.7) as well as the content of masked and exposed SH-groups in sealed and unsealed erythrocyte ghosts were studied as affected by single rapid freezing-thawing. The freezing-thawing procedure resulted in different reactions of membrane-bound enzymes: Na+, K+-ATPase activity is doubled in sealed ghosts and unchanged in unsealed ones. In both types of ghosts the equal decrease in the AChE activity was found to be parallel with the diminution in the content of masked SH-groups but this cannot be referred to exposed SH-groups. The obtained results seem to suggest that the changes in the native conformation of membrane catalytic proteins resulted from cryodamage are responsible for the lowered aChE activity; the primary cause of increase Na+, K+-ATPase activity is due to the changes in the permeability and integrity of erythrocyte membrane, which are followed by the greater accessibility of the substrate (ATP) to the enzyme.
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PMID:[Changes in Na+, K+-ATPase and acetylcholinesterase activity in red cell membranes after freezing-thawing]. 626 9

Erythrocyte ghost preparations have been prepared from blood of Duchenne patients (DMD), female carriers of the disease and controls. Arrhenius plots of Na+, K+-ATPase activity of these membrane preparations show a biphasic response for controls. For 75% of DMD and carriers the response is monophasic. This is not an inherent property of the membrane since it can vary over time in the one individual and it can be induced in normal membranes by preincubation with DMD plasma. Arrhenius plots of AChE activity showed no such difference between the three sources of blood.
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PMID:Thermodynamic behaviour of membrane enzymes in Duchenne muscular dystrophy. 630 27

1. Two membrane fractions were separated from rabbit white muscle SR by discontinuous sucrose gradient. 2. Both crude and membrane fractions were shown to contain AChE, Ca2+-stimulated and Ca2+- independent ATPase activities. 3. 1% W/V Triton X-100 solubilized most of the AChE and Ca2+-stimulated ATPase but the Ca2+- independent ATPase was poorly solubilized. 4. AChE was sensitive to BW284c51, non-sensitive to ethopropazine and presented inhibition by excess of the substrate, ATCh. 5. Polyacrylamide gel electrophoresis from Triton-treated crude SR revealed several bands of AChE and ATPase activities. 6. SDS-gel electrophoresis from crude SR showed two polypeptides specifically labelled with [3H]DFP.
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PMID:Acetylcholinesterase from sarcoplasmic reticulum of white muscle. 710 63


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