Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

---

Query: EC:3.6.1.3 (ATPase)
65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

In the bilaterally growing DBD sensitive Yoshida tumours deformed nuclear divisions necrosis of the majority of the tumour and appearance of giant cells can be observed due to the single administration of the LD50 of the preparation. The histochemical activity of the LDH the activity alcalyc Adenosine triphosphatase and the nonspecific alcalyc phosphatase become negative while the acidic phosphatase's activity does increase after the administration of the LD25 of the preparation appearance of giant cells are very marked more than 60% of tumours cells are polynuction. The activity of the alcalyc ATP-ase and nonspecific phosphatase decrose's after a transitory increase and become negative while the acid phosphatase's activity increases. In the case of the DBD resistant tumours the morphological and histochemical alternations due to LD50 of the preparation are much slighter and their timecourse is shorter. No morphological and histochemical changes are observed after the administration of LD25 of the preparation.
...
PMID:[Cytomorphological and enzyme histological studies of bilaterally inoculated Dibromdulcit-sensitive and-resistent Yoshida tumors]. 101 92

Ultrastructural and histochemical changes of skeletal muscle were studied in three patients affected with gas gangrene. There was complete lack of the phosphorylase, succinate dehydrogenase and adenosine triphosphatase activities in the affected muscles of all the patients. In unaffected muscles these enzymes showed weaker activities than in norm. The lactate dehydrogenase activity, especially the heart type isozyme (LDH-1 or H4) proved less sensitive to the effect of clostridial toxin. A general increase in the acid phosphatase activity was found both in affected and in unaffected muscles. On electron microscopic examination damage to sarcolemmal membrane and disintegration of myofilaments was seen. The mitochondria were swollen and their cristae distorted and fragmented.
...
PMID:Histochemical and electron microscopical studies of skeletal muscle affected by gas gangrene. 120 13

Plasmin activity in the tear fluid of the rabbit eye was examined during the wearing of soft contact lenses (SCL) and compared with the occurrence of corneal disturbances assessed in cryostat sections. Plasmin activity was determined with a semiquantitative method using dry punches of filter paper previously soaked in 0.1 M Tris-HCl buffer solution containing mmol/l D-Val-Leu-Lys-FCA (trifluoromethylaminocoumarine), pH 7.2. Punches were applied to the corneal surface for 5 s (tear collection) and incubated in wet chamber. The time of appearance of the bright yellow fluorescence in UV light was recorded and taken as a measure of plasmin activity. For calibration punches soaked in solutions containing plasmin in various concentrations, and processed in the same manner were used. Changes in the cornea were examined histochemically using methods of choice for acid glycosidases, proteases, dehydrogenases, and Na(+)-K(+)-ATPase. SCL with high and low water content were worn in rabbits in 1, 2, 4, 7, 14, 21 and 28 days. Decreased activity of Na(+)-K(+)-ATPase, GGT, and SDH in the corneal endothelium and epithelium were not accompanied by detectable plasmin activity in the tear fluid. Pronounced damage of the corneal epithelium (increased activities of acid glycosidases, acid proteases, LDH, markedly decreased activity of SDH) was accompanied by low concentration of plasmin (0.4-1.0 micrograms/ml) in the tear fluid. Middle activity of plasmin (1.0-2.0 micrograms/ml) was detectable when PMNs were present in the corneal stroma. High plasmin activity (2.0-3.0 micrograms/ml) correlated with corneal ulceration and vascularization.(ABSTRACT TRUNCATED AT 250 WORDS)
...
PMID:Histochemical changes in the rabbit cornea and plasmin activity in the tear fluid during contact lens wear. Favourable influence of protease inhibitors (aprotinin, PC5, elastatinal). 137 62

(Diphenylphosphinodithioato) diphenylantimony (III), Ph2SbS2PPh2 (1) and (diisopropylphosphorodithioato) diphenylantimony(III), Ph2SbS2P(OPri)2 (2) were tested in vivo in male AKR mice and in vitro against Ehrlich ascites tumor cells. Both compounds exhibited dose-dependent inhibitory effects on in vivo tumor growth and on in vitro cell proliferation, cell viability, respiration and protein synthesis. The activities of some enzymes involved in energetic metabolism (Ca-ATPase, LDH, G6Pase) were exacerbated in vitro. The inhibitory effects could be related to the imbalance between ATP-producing and ATP-consuming processes in Ehrlich ascites tumor cells and also to their cell-cycle specificities.
...
PMID:Antitumor organometallics. II. Inhibitory effects of two diphenyl-antimony (III) dithiophosphorus derivatives on in vitro and in vivo Ehrlich ascites tumor. 166 68

One hundred and five sexually mature male hamsters were divided in different groups. In the first experiment hamsters were administered gossypol, 10 mg/kg and 20 mg/kg/body weight/day, for twenty and thirty days. In the second experiment hamsters were administered gossypol, 5, 10 and 20 mg/kg/body weight/day, for sixty days. In the third experiment, hamsters were administered gossypol 5 mg, 10 mg, 20 mg and 40 mg/kg body weight/day for 45 days. Animals in all the groups were given gossypol by oral intubation every day. No significant effect on the body weight of hamsters following gossypol treatment was observed. At low doses the weights of testis and accessory sex organs were not statistically different from those of the controls. A significant decrease in testis and epididymis weight was however observed following high doses of gossypol. Low doses of gossypol treatment did not affect the motility of the vas deferens spermatozoa. The vas deferens spermatozoa were however immotile after 40 mg/kg/day gossypol treatment. Gossypol treatment induced a series of histological changes in the seminiferous epithelium of the hamster testis. The earliest sign of drug effect was seen in spermatids and with the increase in doses the effects became more pronounced and extended to the spermatocytes. At 40 mg/kg dose an almost complete arrest of spermatogenesis was observed. Quantitatively, the ratio of pachytene spermatocytes: resting spermatocytes and step 7 spermatids: pachytene spermatocytes decreased significantly. The step 7 spermatids did not mature to step 19 spermatids at all. Histochemically activities of ATPase, SDH and LDH decreased with the increasing doses of gossypol, the activity of 3B hydroxysteroid dehydrogenase was not affected by gossypol treatment. In testis the glucose-6-phosphatase activity was not affected significantly but the activities of fructose 1, 6-diphosphatase and glucose-6-phosphate isomerase decreased significantly with the increasing doses of gossypol. Amylase activity rose significantly at higher doses. Marked changes in LDH and LDH-X were however observed with the increase in gossypol dose. In liver the activity of glucose-6-phosphatase increased significantly while the activities of fructose 1, 6-diphosphatase, glucose-6-phosphate isomerase and amylase were not affected following gossypol treatment. The glycogen contents however increased significantly following high doses of gossypol. No changes in testosterone production and plasma levels of testosterone were observed following gossypol treatment.
...
PMID:Response of hamster to the antifertility effect of gossypol. 170 27

In LLC-PK1 cells exposed to patulin (50 microM), lipid peroxidation, abrupt calcium influx, extensive blebbing, and total LDH release appeared to be serially connected events with each representing a step in the loss of structural integrity of the plasma membrane. The aforementioned patulin-induced events were prevented by concurrent incubation with butylated hydroxytoluene, deferoxamine, and cyclopiazonic acid, a fungal metabolite. Patulin also caused depletion of nonprotein sulfhydryls, increased 86Rb+ efflux, dome collapse, and eventually the loss of cell viability. These events were not prevented by antioxidants, results consistent with the hypothesis that they were also serially connected but occurring parallel to those previously mentioned. The earliest events observed in patulin-treated cells were the decrease in nonprotein sulfhydryls and increase in 86Rb+ efflux (5 min) which occurred before statistically significant alterations in protein-bound sulfhydryls. The increased potassium efflux (86Rb+ efflux) occurred via a pathway distinct from BaCl2, quinine, or tetraethylammonium sensitive potassium channels. This is the first published report of the antioxidant activity of indole tetramic acids (cyclopiazonic acid and cyclopiazonic acid imine). The protective effect of tetramic acids in LLC-PK1 cells was restricted to indole tetramic acids, and their prevention of lipid peroxidation did not involve iron chelation. The results of this study demonstrate that cyclopiazonic acid is a potent inhibitor of azide-insensitive, ATP-dependent, a23187-sensitive calcium uptake by the lysate of LLC-PK1 cells. This result is consistent with the hypothesis that the endoplasmic reticulum calcium transport ATPase is a sensitive target for cyclopiazonic acid in LLC-PK1 cells. These findings raise the interesting possibility that the antioxidant activity of indole tetramic acids may involve multiple novel mechanisms: surface charge alterations on the cytoplasmic surface of plasma membranes, alterations in calcium permeability in the plasma and endoplasmic reticulum membrane, and inhibition of the calcium-dependent ATPase of the endoplasmic reticulum.
...
PMID:The mechanism of patulin's cytotoxicity and the antioxidant activity of indole tetramic acids. 203 42

The Ca paradox resulted in marked inhibition, up to disappearance, of histochemically studied enzyme activities (SDH, LDH, beta-HBDH, phosphorylase and ATPase) in the subepicardial layer of the myocardium. In the subendocardial region there was only a small decrease. These transmural differences correlated well with ultrastructural changes. It is assumed that the heterogeneity in transmural distribution of injury is the result of transmural differences in coronary flow.
...
PMID:Transmural non-homogeneity of calcium-induced heart injury. 214 54

Eighty male rats were grouped into 8 groups of 10 animals each. Animals in groups I-IV were given gossypol (40 mg/kg/day) for 7, 14, 21 and 28 days respectively. Animals of groups V-VIII served as respective controls for groups I-IV. Marked changes in the activities of ATPase and SDH were observed following drug treatment. Decrease in the activity of testis LDH was evident even after 7 days of drug treatment. Activities of B-galactosidase, Glucose-6-phosphatase and Fructose-1-6-diphosphatase were not affected by gossypol treatment. Glycogen contents in testis were not different from those of the controls. A significant decrease in the tubular diameter and germinal height of the seminiferous tubules was observed after 21 days of drug treatment. Quantitative analysis of spermatogenic elements revealed marked decrease in the ratios of resting spermatocyte. A type spermatogonia, pachytene spermatocyte/resting spermatocyte, and stage 19 spermatids/stage 7 spermatids after 7 days of drug treatment. A progressive decrease in the ratios of these cell types was observed as the duration of the drug treatment was extended. Liver enzymes (except SDH and LDH after 28 days of drug treatment) were not affected by gossypol treatment. Our data strongly suggest that degenerative changes in the testis start after one week of drug administration. The histological changes visible at light microscopy level start appearing after 14 days of drug treatment.
...
PMID:Early events in rat testis after gossypol administration. 215 Jan 45

Administration of beta- and gamma-isomers of hexachlorocyclohexane (HCH) at 800 ppm dietary level for 2 weeks to albino rats produced noticeable hepatocellular damage as indicated by elevations in serum aminotransferases and decreases in hepatic soluble enzymes. Although serum total LDH activity was not altered, the LD5 isoenzyme was proportionately higher in the HCH isomers treated animals. Treatment of rats with beta- and gamma-isomers of HCH increased the hepatic glucose-6-phosphate dehydrogenase and aldolase activities suggesting a higher rate of glucose oxidation. Liver glucose-6-phosphatase activity was decreased in these animals indicating inactivation of gluconeogenesis in liver. Dietary beta- and gamma-HCH decreased the liver mitochondrial DNP/Mg++/Ca++-activated ATPases thus affecting the energy metabolism. An unaltered ratio of DNP/Mg++-ATPase, a study of swelling pattern of hepatic mitochondria, and NAD+ permeability test suggested the maintenance of structural integrity of mitochondrial membrane in these pesticide fed animals. Liver microsomal Na+,K+-ATPases were lower in these animals.
...
PMID:Biochemical changes produced by beta- and gamma-hexachlorocyclohexane isomers in albino rats. 246 8

We studied the testis of Wistar rats weighing 280-300 gms. following the administration of a single, acute intracardiac dose of methionine-enkephalin (100 microliters of 50% met-enkephalin solution), or a chronic intramuscular dose (50 microliters of 40% met-enkephalin solution). Rats were sacrificed at 15, 30 and 60 minutes following acute injection. Those on chronic treatment were injected once daily for 10 or 20 days. For the study, we utilized 105 male Wistar rats; 30 comprised the control group, and 75 comprised the study group. The following staining methods were used: 1) succinate dehydrogenase, 2) lactate dehydrogenase, 3) ATPase, 4) acid phosphatase, 5) alkaline phosphatase. We observed marked histoenzymological changes in the rat testis. Particularly noteworthy was a marked change in the energy pathways consisting of a decreased activity of aerobic pathways (decreased SDH activity), increased anaerobic activity (increased LDH activity), and consequently, decreased cellular energy stores (decreased ATPase activity). Similarly, changes were observed in other nonspecific enzymes that led to a fall in acid phosphatase activity and a rise in alkaline phosphatase activity.
...
PMID:[Effects of met-enkephalin on the testis. III. Histoenzymatic study]. 253 59


<< Previous 1 2 3 4 5 6 7 8 9 10 Next >>

---