Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.1.3 (
ATPase
)
65,361
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
RNA helicases contribute to diverse aspects of RNA metabolism through their functions in re-arranging RNA structures. Identification of the remodelling targets of RNA helicases is a critical step in elucidating their cellular functions. Here, we show that, in contrast to many other ribosome biogenesis factors, the DExD box
ATPase
DDX55
predominantly localizes to the nucleoplasm and we identify a nuclear localization signal within the C-terminal region of the protein.
DDX55
associates with pre-ribosomal subunits in human cells and is required for maturation of large subunit pre-rRNAs. Interestingly,
in vitro
analyses show that
DDX55
selectively associates with double-stranded RNA substrates, which also stimulate its
ATPase
activity, and our data suggest that the C-terminal region of
DDX55
contributes to this substrate specificity. The C-terminal region of
DDX55
is also necessary for recruitment of the helicase to pre-ribosomes and, using
in vivo
crosslinking, we reveal a binding site for
DDX55
in helix H62 of the 28S ribosomal RNA. Taken together, these data highlight the importance of the C-terminal region of
DDX55
in substrate specificity and recruitment, and identify domain IV as a potential remodelling target of
DDX55
during LSU biogenesis.
...
PMID:The DExD box ATPase DDX55 is recruited to domain IV of the 28S ribosomal RNA by its C-terminal region. 3304
