Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

A nontoxic and simple staining method for the detection of DNA in agarose gels is described. After eletrophoretic separation, the gels were stained with 5 microg/ml of berberine (BB) prepared in distilled water and then the gels were soaked in 20 microg/ml of aqueous Mordant Yellow 3R (MY3R) solution. Employment of MY3R as a counterion dye efficiently quenched unwanted background fluorescence of BB. This method can detect as little as 10 ng of plasma membrane H(+)-ATPase cDNA obtained from Arabidopsis thaliana L. (AHA1, 3.2 kb) under a long wavelength of UV irradiation (366 nm) within 1 h.
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PMID:Detection of DNA in agarose gels using berberine and Mordant Yellow 3R. 1041 96