Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.1.3 (
ATPase
)
65,361
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Post-germination plant growth depends on the regulation of reactive oxygen species (ROS) metabolism, spatiotemporal pH changes and Ca
+2
homeostasis, whose potential integration has been studied during Vigna radiata (L.) Wilczek root growth. The dissipation of proton (H
+
) gradients across plasma membrane (PM) by CCCP (protonophore) and the inhibition of PM H
+
-
ATPase
by sodium orthovanadate repressed SOD (superoxide dismutase; EC 1.15.1.1) activity as revealed by spectrophotometric and native PAGE assay results. Similar results derived from treatment with DPI (NADPH oxidase inhibitor) and Tiron (O
2
- scavenger) denote a functional synchronization of SOD, PM H
+
-
ATPase
and NOX, as the latter two enzymes are substrate sources for SOD (H
+
and O
2
-, respectively) and are involved in a feed-forward loop. After SOD inactivation, a decline in apoplastic H
2
O
2
content was observed in each treatment group, emerging as a possible cause of the diminution of class III peroxidase (
Prx
; EC 1.11.1.7), which utilizes H
2
O
2
as a substrate. In agreement with the pivotal role of Ca
+2
in PM H
+
-
ATPase
and NOX activation, Ca
+2
homeostasis antagonists, i.e., LaCl
3
(Ca
+2
channel inhibitor), EGTA (Ca
+2
chelator) and LiCl (endosomal Ca
+2
release blocker), inhibited both SOD and
Prx
. Finally, a drastic reduction in apoplastic OH (hydroxyl radical) concentrations (induced by each treatment, leading to
Prx
inhibition) was observed via fluorometric analysis. A consequential inhibition of root growth observed under each treatment denotes the importance of the orchestrated functioning of PM H
+
-
ATPase
, NOX, Cu-Zn SOD and
Prx
during root growth. A working model demonstrating postulated enzymatic synchronization with an intervening role of Ca
+2
is proposed.
...
PMID:Orchestration of Cu-Zn SOD and class III peroxidase with upstream interplay between NADPH oxidase and PM H
+
-ATPase mediates root growth in Vigna radiata (L.) Wilczek. 3053 11
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