Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Campbell, J. J. R. (The University of British Columbia, Vancouver, B.C., Canada), Loretta A. Hogg, and G. A. Strasdine. Enzyme distribution in Pseudomonas aeruginosa. J. Bacteriol. 83:1155-1160. 1962.-Previous studies on the distribution of enzymes in bacteria have indicated that, although individual enzymes were predominantly associated with a particular cellular structure, nevertheless some of the enzyme appeared to be present in all cellular fractions. In the present work with Pseudomonas aeruginosa, it was shown that, in general, an enzyme is present in only one cellular component. Hexokinase, glucose-6-phosphate dehydrogenase, 6-phosphogluconic dehydrogenase, gluconic dehydrogenase, malic dehydrogenase, fumarase, isocitric dehydrogenase, isocitritase, and catalase were detected only in the soluble cytoplasm of the cell. Glucose oxidase and succinic dehydrogenase were detected only in the "ghost" fraction. Diphosphopyridine nucleotide oxidase was present in both "ghost" and ribosomal fractions but was most concentrated in the "ghost". Although adenylic kinase was found to be present in all fractions, it was possible to fractionate cells so that almost all of the activity was associated with the soluble cytoplasm a minor amount being associated with the "ghost." Adenosine triphosphatase was most concentrated in the "ghost" but appreciable activity appeared in the cytoplasm. Polynucleotide phosphorylase appeared to be the only enzyme that was convincingly associated with the ribosomes. However, a small amount of activity was associated with the soluble cytoplasm and with the "ghosts."
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PMID:Enzyme distribution in Pseudomonas aeruginosa. 1387 40

Subtractive hybridisation was used to target novel genes involved in the mycoparasitic interaction of the biocontrol agent Trichoderma hamatum with the phytopathogen Sclerotinia sclerotiorum. Nineteen novel T. hamatum genes were identified that showed increased expression during mycoparasitism compared to a T. hamatum control. Sequence analysis revealed some cDNA fragments had similarity to known fungal or bacterial genes whereas others had no similarity to any genes previously described. Only one of the novel genes has been characterised in another Trichoderma species, the Trichoderma reesei hex1gene. The proteins encoded by the novel genes included three monooxygenases, a metalloendopeptidase, a gluconate dehydrogenase, an endonuclease and a proton ATPase.
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PMID:Identification of novel Trichoderma hamatum genes expressed during mycoparasitism using subtractive hybridisation. 1614 62