Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Colchicine is known to affect secretory, transport, and degradative functions of ameloblasts. The effects of colchicine on membrane-associated calcium and Ca2+,Mg2(+)-ATPase in secretory and maturation ameloblasts were investigated cytochemically. The pyroantimonate (PPA) method was used for localizing calcium and a modified Wachstein-Meisel medium was used to localize Ca2+,Mg2(+)-ATPase. Sections representing secretory and early maturation stages were examined by transmission electron microscopy. Morphological changes induced by colchicine included dislocated organelles and other well-established reactions to such anti-microtubule drugs. Calcium pyroantimonate (Ca-PA) deposits in most ameloblast types were markedly reduced, with the greater reduction occurring in those cells more severely altered morphologically. However, the cell membranes of both control and experimental smooth-ended maturation ameloblasts were essentially devoid of Ca-PA. The normal distribution and intensity of Ca2+,Mg2(+)-ATPase was not affected by colchicine. Because the observed reduction of membrane-associated calcium is apparently not mediated by Ca2+,Mg2(+)-ATPase in this case, other aspects of the calcium regulating system of ameloblasts are apparently targeted by colchicine.
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PMID:Cytochemical localization of calcium and Ca2+,Mg2(+)-adenosine triphosphatase in colchicine-altered rat incisor ameloblasts. 214 64

Vegetative-bud dormancy in peach (Prunus persica L. Batsch) trees is known to be correlated, at least partially, with properties of the underlying bud tissues during winter. Variations in the activity and amount of plasma-membrane H -ATPase were observed. A full-length cDNA, PPA2 (Prunus persica H+-ATPase 2) and three partial cDNAs (PPA1, PPA3 and PPA4) for the plasma-membrane H+-ATPase from peach trees were isolated by reverse transcription (RT)-coupled rapid amplification of cDNA ends (RACE) polymerase chain reaction (PCR). The accumulation of plasma membrane H+-ATPase transcripts was then studied in vegetative buds during dormancy and breaking of dormancy. Competitive RT-PCR analysis revealed that, during dormancy, the plasma membrane H+-ATPase transcripts were higher in the tissues underlying the buds than in the buds themselves. After dormancy release, the level of PPA1, 2, 3 mRNA increased, whereas the level of PPA4 decreased in the buds. When trees were kept in a greenhouse (i.e. sheltered from chilling), no accumulation of PPA mRNA could be detected. These results suggest that there is a differential accumulation of H+-ATPase mRNA between the bud and the underlying bud tissues during dormancy, and that chilling could act as a decisive factor.
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PMID:Differential expression of four members of the H+-ATPase gene family during dormancy of vegetative buds of peach trees. 1152 20