Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.1.3 (
ATPase
)
65,361
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Using a gel-overlay technique of biotinylated calmodulin (CaM), we showed that maize cytosolic Hsp70 protein could bind to CaM in the presence of 1 mM CaCl2. The purified maize cytosolic Hsp70 inhibited the activity of CaM-dependent
NADK
in a concentration-dependent manner. A synthetic peptide, which possesses the 21 amino acid sequence, PRALRRLRTACERAKRTLSST, at positions 261-281 in maize cytosolic Hsp70, could associate with CaM in the presence of 1 mM calcium. The synthetic peptide inhibited CaM-dependent
NADK
activity and PDE activity. This indicates that the 21-amino acid sequence at positions 261-281 is the CaM-binding site. The binding of CaM to Hsp70 inhibited the
ATPase
activity of Hsp70. The possible regulator function of Hsp70 in cell signaling events in response to heat stress is discussed.
...
PMID:Binding of the maize cytosolic Hsp70 to calmodulin, and identification of calmodulin-binding site in Hsp70. 1094 51
Effects of propyl gallate on metabolisms of respiration and energy of harvested 'Fuyan' longans and its relationship to pericarp browning were investigated. Compared to control longans, propyl gallate could reduce ascorbic acid oxidase (AAO) activity, lower cytochrome C oxidase (CCO) activity during early-storage and mid-storage, increase
NADK
activity, elevate contents of NADP and NADPH, decrease contents of NAD and NADH, in addition, lower the decreases of ATP content and energy charge (E.C.), increase activities of mitochondrial H
+
-
ATPase
, Ca
2+
-
ATPase
and Mg
2+
-
ATPase
during early-storage and mid-storage. Above results suggested that propyl gallate-retarded browning development in pericarp of harvested longans was resulted from decreases in activities of respiratory terminal oxidases like CCO and AAO, increase in proportion of pentose phosphate pathway (PPP) to Embden-Meyerhof pathway (EMP) and tricarboxylic acid (TCA) cycle, and maintenance of mitochondrial integrity via retaining higher levels of ATP content and energy charge, as well as higher activities of mitochondrial ATPase.
...
PMID:Application of propyl gallate alleviates pericarp browning in harvested longan fruit by modulating metabolisms of respiration and energy. 2894 53
