Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.6.1.3 (
ATPase
)
65,361
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Recombinant Ca(2+)-
ATPase
from tomato (i.e. LCA1 for Lycopersicon esculentum [Since the identification and naming of LCA1, the scientific name for the tomato has been changed to Solanum lycopersicum.] Ca-
ATPase
) was heterologously expressed in yeast for structure-function characterization. We investigate the differences between plant and animal Ca pumps utilizing comparisons between chicken and rabbit SERCA-type pumps with Arabidopsis (
ECA1
) and tomato plant (LCA1) Ca(2+)-ATPases. Enzyme function was confirmed by the ability of each Ca(2+)-
ATPase
to rescue K616 growth on EGTA-containing agar and directly via in vitro ATP hydrolysis. We found LCA1 to be approximately 300-fold less sensitive to thapsigargin than animal SERCAs, whereas
ECA1
was thapsigargin-resistant. LCA1 showed typical pharmacological sensitivities to cyclopiazonic acid, vanadate, and eosin, consistent with it being a P(IIA)-type Ca(2+)-
ATPase
. Possible amino acid changes responsible for the reduced plant thapsigargin-sensitivity are discussed. We found that LCA1 also complemented K616 yeast growth in the presence of Mn(2+), consistent with moving Mn(2+) into the secretory pathway and functionally compensating for the lack of secretory pathway Ca-ATPases (SPCAs) in plants.
...
PMID:A tomato ER-type Ca2+-ATPase, LCA1, has a low thapsigargin-sensitivity and can transport manganese. 1905 36
Ever since Darwin postulated that the tip of the root is sensitive to moisture differences and that it "transmits an influence to the upper adjoining part, which bends towards the source of moisture" [Darwin C, Darwin F (1880)
The Power of Movement in Plants
, pp 572-574], the signal underlying this tropic response has remained elusive. Using the FRET-based Cameleon Ca
2+
sensor
in planta
, we show that a water potential gradient applied across the root tip generates a slow, long-distance asymmetric cytosolic Ca
2+
signal in the phloem, which peaks at the elongation zone, where it is dispersed laterally and asymmetrically to peripheral cells, where cell elongation occurs. In addition, the MIZ1 protein, whose biochemical function is unknown but is required for root curvature toward water, is indispensable for generating the slow, long-distance Ca
2+
signal. Furthermore, biochemical and genetic manipulations that elevate cytosolic Ca
2+
levels, including mutants of the endoplasmic reticulum (ER) Ca
2+
-
ATPase
isoform
ECA1
, enhance root curvature toward water. Finally, coimmunoprecipitation of plant proteins and functional complementation assays in yeast cells revealed that MIZ1 directly binds to
ECA1
and inhibits its activity. We suggest that the inhibition of
ECA1
by MIZ1 changes the balance between cytosolic Ca
2+
influx and efflux and generates the cytosolic Ca
2+
signal required for water tracking.
...
PMID:MIZ1 regulates ECA1 to generate a slow, long-distance phloem-transmitted Ca
2+
signal essential for root water tracking in
Arabidopsis
. 3001 18
