Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Eight muscle biopsies (3 from the left biceps, 3 from the left gastrocnemius, and 2 from the left quadriceps) of patients with hypothyroid myopathy were studied in the light of the previous literature. Some new observations have been made. First, the percentage of type II fibres is higher than that of type I fibres in all but 1 cases before treatment. Second, 5 of 8 cases before treatment disclosed 'core-like' structures, readily recognized with oxidative enzyme preparations in eccentric positions or in the periphery of type I fibres. They were reactive with myofibrillar adenosine triphosphatase (ATPase) activity and periodic acid-Schiff(PAS) staining, whereas with hematoxylineosin (HE) and modified Gomori trichrome the regions were more strongly reactive than the rest of the fibre. When examined by electron microscopy, within the 'core-like' structures of affected fibres, the A, I, and Z banding pattern was markedly disrupted. These structures disappeared after treatment with L-thyroxine. Third, none of the cases with Hoffmann's syndrome showed individual muscle fibre hypertrophy. Further study of these findings may yield information on clarifying the characteristics of muscle pathology of hypothyroid myopathy.
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PMID:Myopathology of hypothyroid myopathy. Some new observations. 381 67

Sarcoplasmic/endoplasmic reticulum Ca(2+) ATPase (SERCA) pumps play the major role in lowering cytoplasmic calcium concentration in skeletal muscle by catalyzing the ATP-dependent transport of Ca(2+) from the cytosol to the lumen of the sarcoplasmic reticulum (SR). Although SERCA abnormalities have been hypothesized to contribute to the dysregulation of intracellular Ca(2+) homeostasis and signaling in muscle of patients with myotonic dystrophy (DM) and hypothyroid myopathy, the characterization of SERCA pumps remains elusive and their impairment is still unclear. We assessed the activity of SR Ca(2+)-ATPase, expression levels and fiber distribution of SERCA1 and SERCA2, and oligomerization of SERCA1 protein in muscle of patients with DM type 1 and 2, and with hypothyroid myopathy. Our data provide evidence that SR Ca(2+) ATPase activity, protein levels and muscle fiber distribution of total SERCA1 and SERCA2, and SERCA1 oligomerization pattern are similar in patients with both DM1 and DM2, hypothyroid myopathy and in control subjects. We prove that SERCA1b, the neonatal isoform of SERCA1, is expressed at protein level in muscle of patients with DM2 and, in lower amount, of patients with DM1. Our present study demonstrates that SERCA function is not altered in muscle of patients with DM and with hypothyroid myopathy.
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PMID:Characterization of sarcoplasmic reticulum Ca(2+) ATPase pumps in muscle of patients with myotonic dystrophy and with hypothyroid myopathy. 2713 61