Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.6.1.3 (ATPase)
 65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

1. It has been observed previously that gastrocnemius muscles from rats rehabilitated after early nutritional stress have low endurance and oxidative capacity (Raju, 1974). 2. Exercise during rehabilitation reversed the effects of early malnutrition on muscle function and metabolism. The swimming performance of rehabilitated rats was poor and exercise during rehabilitation improved it. 3. The specific activity of gastrocnemius muscle myosin ATP phosphohydrolase (EC 3.6.1.3) was not altered by either early malnutrition or exercise.
 ...
PMID:Effect of exercise during rehabilitation on swimming performance, metabolism and function of muscle in rats. 91 38

Malnutrition has been associated with changes in cardiac metabolism and performance. We have previously reported a diabetic-type cardiomyopathy associated with chronic food restriction and weight loss. Because the creatine-phosphocreatine-creatine kinase system is important in the contractile process, we studied the components of this system in rats fed a food-restricted diet (33% of control animal intake). After 4 weeks of food restriction, total creatine kinase (CK) activities were reduced by 28% in ventricles and by 38% in atria. The CK isoenzymes in the heart were not equally affected. The BB isoenzyme was decreased by 77% and 78%, the MB isoenzyme by 45% and 43%, the MM isoenzyme by 22% and 19% and CKmito by 16% and 15% in ventricles and atria, respectively. In contrast, brain CK activity which is predominantly the BB isoenzyme, was slightly higher in the food-restricted than in control rats. Further studies on ventricular tissue from food-restricted rats revealed a 27% decline in myofibrillar CR activity and a 58% decline in myofibrillar ATPase activity. Phosphocreatine and creatine concentrations were not changed by food restriction, however, ATP was decreased by 23% in ventricles from rats on the restricted diet. Cardiac mitochondrial oxidative phosphorylation was also impaired. State 3 respiration with alpha-ketoglutarate was reduced 20% in the food-restricted heart. These changes are compared to those which we previously observed in the diabetic rat heart and the significance of these findings is discussed.
 ...
PMID:Effect of food restriction on the phosphocreatine energy shuttle components in rat heart. 143 12

To assess whether nonorganic nutritional dwarfing (ND) is accompanied by a biochemical adaptation to reduced nutrient intake, Na+,K(+)-ATPase activity of erythrocytes was examined. The study included 27 children with ND who demonstrated deteriorating linear growth and poor weight gain (caused by self-imposed dietary restrictions), 20 patients who initially exhibited ND growth patterns and subsequently experienced catch-up weight gain and growth after nutritional rehabilitation (NDR), and 32 normally growing children who had familial short stature and/or constitutional growth delay (F/CSS). Na+,K(+)-ATPase activity in erythrocytes was significantly lower in ND patients than in the NDR and F/CSS groups. Furthermore, Na+,K(+)-ATPase activity was positively correlated with incremental body weight gain. Na+,K(+)-ATPase concentrations did not differ significantly with regard to sex, chronological age, bone age, or pubertal status. These data suggest that the growth retardation of ND patients is associated with decreased erythrocyte Na+,K(+)-ATPase activity without other biochemical evidence of malnutrition.
 ...
PMID:Nutritional dwarfing: a growth abnormality associated with reduced erythrocyte Na+,K(+)-ATPase activity. 165 81

Postweaning protein malnutrition imposed on normally weaned or neonatally undernourished rats fed a low-protein diet induced retardation of body and small intestinal growth. A sparing effect on intestinal growth as compared to body growth was observed during protein malnutrition. Postweaning protein malnutrition in normally weaned rats resulted in a significant elevation of specific activities of inositol triphosphatase and phytase in duodenum and jejunum without affecting the activity in ileum. On the other hand, protein malnutrition imposed on neonatally undernourished rats resulted in a significant decrease of enzyme activities in small intestinal segments. These results suggest altered activity of intestinal inositol phosphatase in postweaning protein malnutrition with the direction of effects dependent on the neonatal nutritional status.
 ...
PMID:Effects of postweaning protein malnutrition on intestinal inositol phosphatase activities in normally weaned and neonatally undernourished rats. 216 59

Growing rats and adult weight-stable mice bearing a transplantable methylcholanthrene-induced sarcoma were compared with animals with various states of malnutrition. Heart protein synthesis was measured in vivo. Myocardial RNA, myofibrillar protein composition and the Ca2+-activated ATPase activity in heavy chains of native myosin were measured. 'Fingerprints' were made from myosin by trypsin treatment to evaluate possible structural changes in the protein. Cardiac protein-synthesis rate was decreased by 20% in growing tumour-bearing rats, by 35% in protein-malnourished (rats) and by 47% in starved rats, compared with freely fed controls (P less than 0.05). Adult tumour-bearing mice showed no significant decrease in myocardial protein synthesis. Pair-weighed control mice had significantly depressed heart protein synthesis. Protein translational efficiency was maintained in both tumour-bearing rats and mice, but was decreased in several groups of malnourished control animals. The Ca2+-activated myosin ATPase activity was decreased in all groups of malnourished animals, including tumour-bearing mice and rats, without any evidence of a change in cardiac isomyosin composition. We conclude that loss of cardiac muscle mass in tumour disease is communicated by both depressed synthesis and increased degradation largely owing to anorexia and host malnutrition. Increased adrenergic sensitivity in hearts from tumour-bearing and malnourished animals is not communicated by increased Ca2+-activated ATPase activity. This may be down-regulated in all groups with malnutrition, without any observable alterations in the isomyosin profile.
 ...
PMID:Protein synthesis, myosin ATPase activity and myofibrillar protein composition in hearts from tumour-bearing rats and mice. 248 44

An assessment of mitochondrial bioenergetics in protein-malnourished rats indicates that there is a reduction of brain mitochondrial metabolism in protein malnutrition. Specifically, mitochondria of protein-malnourished offspring of malnourished rats exhibit (i) an enhancement of state 4 respiration, (ii) a reduction of ADP-stimulated state 3 respiration, (iii) a decrease in ADP:O ratios, (iv) reductions of FCCP-induced ATPase action and in the rates of proton and calcium ion translocation, and (v) diminished activities of redox enzymes. In general, these changes are less pronounced in malnourished weanling rats born of healthy dams. Although the exact molecular mechanism of these defects is not yet known, the alterations are definitely caused by diet-induced changes in the structure and integrity of inner mitochondrial membrane components.
 ...
PMID:Changes in brain mitochondrial bioenergetics in protein-deficient rats. 251 64

The relationship between undernutrition-induced alterations in some myelin membrane-bound enzyme activities and phospholipid fatty acid composition of this membrane was ascertained in developing rat brains. Undernutrition was imposed in pregnant dams through gestation and lactation, (last 10 days of gestation, and through lactation) by feed restriction. Experimental groups of animals received 50% of the amount of diet consumed by controls. Pups born to these mother rats were killed at day 7, 14 or 21 of postnatal age. Myelin membrane was isolated from the major regions of the brain, and analysed for phospholipid fatty acid profiles, phosphoinositides and ganglioside species. While there were no diet-related differences in the activities of 5'-nucleotidase (EC 3.1.3.5), myelin phospholipids from cerebella and brain stems of experimental rats exhibited lowered proportions of the long-chain polyunsaturated fatty acids, C20:4 (n - 6) and C22:6 (n - 3) concomitant with elevated activities of ouabain-sensitive Na+/K+-ATPase (EC 3.6.1.4). Levels of diphosphoinositide, triphosphoinositide and trisialogangliosides also decreased in myelin from brains of experimental animals. These results suggest a relationship between myelin phospholipid fatty acid profiles as indicators of membrane unsaturation, and the possibility of allosteric modification of Na+/K+-ATPase activity.
 ...
PMID:Activity of myelin membrane Na+/K+-ATPase and 5'-nucleotidase in relation to phospholipid acyl profiles, ganglioside composition and phosphoinositides in developing brains of undernourished rats. 254 71

Dietary manipulations involving high carbohydrate feeding increase V1 cardiac myosin isoform expression in hormonally deficient rats. The purpose of this study was to determine if extremes in dietary carbohydrate availability could alter cardiac myosin isoform patterns in normal weanling and adult rats. Three and six weeks of dietary manipulations (either high or low carbohydrate diets) failed to change calcium-activated myofibril ATPase activity, calcium regulated myofibril ATPase activity, or the myosin isoform distribution in the adult. In contrast, a four week, high carbohydrate diet reduced calcium activated myosin ATPase activity by 33%, calcium regulated myofibril ATPase activity by 10%, and V1 isoform expression by 66% in weanling rats. Although the low carbohydrate diet caused no change in the myosin ATPase properties, it decreased V1 isoform expression by 17%. These results show that carbohydrate availability can alter cardiac myosin isoform expression in normal rats, but only at weanling age. The reason for this age-related contrast in response to dietary manipulations is unknown at this stage. The dietary manipulations may have acted directly on the heart by creating a state of malnutrition, or indirectly, by altering some developmental process which links maturation of the sympathetic nervous system with myosin isoform expression.
 ...
PMID:Differential effects of carbohydrate intake on cardiac myosin isoform expression in normal weanling and adult rats. 296 58

The influence of vitamin D and C deficiency on the kinetic parameters of sucrase and alkali phosphatase activities was studied in the microsomal fraction of the small intestinal mucosa of guinea pigs. It was found that Km values for these enzymes did not depend on the animal providing with these vitamins. Deficiency of one of these vitamins did not influence sucrase activity, however, simultaneous elimination of vitamins D and C resulted in the activity rise by 92%. Alkali phosphatase and Ca-ATPase activities proved to be similarly dependent on providing with vitamin D in the presence of vitamin C in the ration, while in the absence of vitamin C this dependence was not observed.
 ...
PMID:[Enzymatic activity of the microsomal fraction of the mucosa of the small intestine in guinea pigs with vitamin D and C deficiencies]. 296 18

The status of the erythrocyte sodium pump was evaluated in a group of patients suffering from anorexia nervosa and a group of healthy female control subjects. Anorectic patients showed significantly higher mean values of digoxin-binding sites/cell (ie, the number of Na-K-ATPase units) with respect to control subjects while no differences were found in the specific 86Rb uptake (which reflects the Na-K-ATPase activity) between the two groups. A significant correlation was found between relative weight and the number of Na-K-ATPase pump units (r = -0.66; P less than 0.0001). Anorectic patients showed lower serum T3 concentrations (71.3 +/- 53 ng/dL) with respect to control subjects (100.8 +/- 4.7 ng/dL; P less than 0.0005) and a significant negative correlation between T3 levels and the number of pump units (r = -0.52; P less than 0.003) was found. Our study therefore shows that the erythrocyte Na-K pump may be altered in several anorectic patients. We suggest that this feature could be interrelated with the degree of underweight and/or malnutrition.
 ...
PMID:Altered erythrocyte Na-K pump in anorectic patients. 298 54


1 2 3 4 5 6 7 Next >>