Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound   Target Concepts: Gene/Protein Disease Symptom Drug Enzyme Compound

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Query: EC:3.6.1.3 (ATPase)
65,361 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Immunohistological analysis of experimental gingivitis in humans was carried out to provide a baseline for the study of immunoregulatory mechanisms in chronic inflammatory periodontal disease. Using a panel of monoclonal antibodies in an avidin biotin immunoperoxidase technique, T cell subsets were identified and the pattern of Class II major histocompatibility complex (MHC) antigens determined. Twenty third-year dental students took part in the study. Following the cessation of oral hygiene procedures, gingival biopsies were taken from each of five students at days 0, 4, 8 and 21 during the development of the inflammatory lesion. Each student had one biopsy which healed uneventfully. The T4:T8 ratio showed only slight variation over the time course of the lesion varying from 2.18:1 at day 0 to 2.48:1 at day 4. At all stages the T cells displayed both HLA-DR and HLA-DQ antigens, but less than 10% had detectable IL-2 receptors. The predominant macrophage population was acid phosphatase + ve, adenosine triphosphatase -ve, HLA-DR+ and HLA-DQ+ antigens suggesting an activated phagocytic population. During the development of the lesion, the number of intraepithelial Langerhans cells (T6+) increased but there appeared to be a discrepancy between HLA-DR and HLA-DQ expression on these cells. Similarly, the keratinocytes expressed HLA-DR but failed to express HLA-DQ at any stage. These results suggest that the developing gingival lesion is a well controlled lesion and follows a similar pattern to a controlled delayed type hypersensitivity (DTH) response.
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PMID:Immunohistological analysis of experimental gingivitis in humans. 328 Jan 78

The epidermal Langerhans cells has been keeping, most of its secrets for about a hundred years since its first description in 1868. Its characterization in electron microscopy by Birbeck and then the use of histoenzymology (ATPase) and monoclonal antibodies (against HLA-DR, T6, LCA, C3b...) brought a new great interest on its study. The Langerhans cell must play an important immunologic role in the epidermis because of its responsibility in the antigenic information of the lymphocytes; in this way it may represent the specific epidermal differentiation of the monocyte-macrophage cell type, which are all bone-marrow derived cells. The epidermal Langerhans cell is one of the most important links in the elicitation of contact dermatitis. Recent trends about its metabolism, antigens and functions are reviewed and discussed.
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PMID:[The Langerhans cell. Recent immunologic achievements]. 333 Oct 87

A 58-year-old man presented with an unusual sarcoma of the cervical lymph node. The tumor also involved the mesenteric lymph node and jejunum. Tumor cells possessed intracytoplasmic S100 protein, Leu-3a (T4), and HLA-DR antigens. The neoplastic cells also showed membranous ATPase activity. LeuM1, T6, Leu1, Leu2a, B1, lysozyme, and immunoglobulin were not recognized. Their fine structure was similar to that of interdigitating cells. These data are consistent with derivation from lymph node interdigitating reticulum cell.
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PMID:Interdigitating cell sarcoma. A morphologic, immunohistologic, and enzyme-histochemical study. 333 24

Immunocytochemical and histochemical properties of macrophages present in the subcutaneous chronic inflammatory responses surrounding adult Onchocerca volvulus (nodules) in human tissues were examined. Macrophages with strong non-specific esterase (NSE) and acid phosphatase (AcPase) activities but weak adenosine triphosphatase (ATPase) activity and HLA-DR expression (NSE+++, AcPase+++, ATPase-/+, HLA-DR-/+) were present in the centre of nodules. Many of the cells adhering to the surface of worms were NSE+++, AcPase+++, ATPase-, HLA-DR+++. The inner zone of the fibrous capsule of nodules contained macrophages with the profile NSE+++, AcPase-, ATPase-/+, HLA-DR-/+. A fourth type, NSE+++, AcPase-/+, ATPase-/+, HLA-DR+++, was located in the outer zone of the capsule, frequently within perivascular accumulations of macrophages, lymphocytes and plasma cells. Active fibroblasts were identified at the inner edge of the fibrous capsule by alkaline phosphatase staining. A feature of all nodules examined was the presence of lipid-filled macrophages, demonstrated by Oil Red O stain; these cells were usually situated in zones adjacent to the centre of nodules, and were of the NSE++, AcPase++, ATPase-/+, HLA-DR-/+ type. Lipid accumulation was not found to be related to the clinical status of the patients studied. The origin and functional significance of this lipid is unknown.
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PMID:A histocytochemical study of the macrophages present in tissue responses to adult Onchocerca volvulus. 344 Jul 61

A large number of cells containing subunit a of blood coagulation Factor XIII (FXIII) was detected by immunoperoxidase staining in lymph nodes with Hodgkin's disease. These relatively large, multipolar, mononuclear cells were often found in the immediate vicinity of malignant Hodgkin's cells. Intensive characterization of these cells carried out by immunofluorescent and enzymecytochemical techniques in double- and triple-labelling systems on the same sections clearly demonstrated that they represent tumour-associated macrophages (TAMs). FXIII containing-cells showed alpha-naphtyl acetate esterase (ANAE) positivity, and were labelled by monoclonal anti-Leu M3 antibody, a monocyte/macrophage marker, but not at all or only very weakly by anti-HLA-DR. Neither alkaline phosphatase (ALP) nor adenosine triphosphatase (ATPase) activity could be detected in these cells and surprisingly, they were consistently negative for acid phosphatase (AcP) as well. The presence of FXIII subunit a in tumour-associated macrophages suggests that this cell type might have an important role in the stabilization of fibrin deposits around tumour cells.
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PMID:Characterization of factor XIII containing-macrophages in lymph nodes with Hodgkin's disease. 355 91

The effects of 3 daily, 7-min exposures to artificial ultraviolet light (UVL), designed to simulate natural sunlight, on epidermal Langerhans cells (LC) and melanocytes were studied in 17 healthy Australian volunteers of differing skin pigmentation. Six were of Celtic, 6 of Asian and 5 of Aboriginal descent. LC were visualized using the immunofluorescence method for HLA-DR and T6 antigens, and the histochemical method for ATPase. UVL induced a transient reduction in the LC population density and an increase in the number of melanocytes in all subject groups. The reduction in number of immunocompetent LC or the disruption of their surface markers was greatest in the Celtic subjects, who had the fairest skin, and least in the Aboriginal and Asian subjects, who had the darkest skin. However, neither the inherently dark skin pigmentation nor the UVL-induced increase in pigmentation were sufficient to prevent the depletion of immunocompetent epidermal LC. Non-dendritic, rounded cells and very large dendritic, non-LC, which were present in the epidermis of some subjects, were stimulated to increase in number by exposure to UVL. The identity and function of these cells is uncertain and they require further investigation.
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PMID:Effects of exposure to ultraviolet light on epidermal Langerhans cells and melanocytes in Australians of aboriginal, Asian and Celtic descent. 361 49

The authors describe a 63-year-old woman who developed a histologically distinctive malignant cutaneous neoplasm composed of large pleomorphic cells with abundant cytoplasm and multilobate, often clefted nuclei that occasionally contained small nucleoli. This neoplastic cell population metastasized to a regional lymph node already involved by a B-cell derived chronic lymphocytic leukemia expressing surface IgMk, BA-1, and OKT1. The large metastatic tumor cells lacked surface immunoglobulin, B-lymphocyte associated antigen BA-1, T-lymphocyte associated antigens OKT1 and OKT3, and the monocyte/macrophage markers lysozyme and alpha 1-antichymotrypsin. These tumor cells expressed HLA-DR antigens, adenosine triphosphatase (ATPase), OKT6, and contained S-100 protein, i.e., they expressed the phenotype peculiar to epidermal Langerhans cells. The typical clinical and histologic features of Histiocytosis X were absent. Thus, this case appears to represent a distinctive cutaneous neoplasm composed entirely of malignant cells of dendritic cell origin which, by immunophenotypic and histochemical analysis, appear to be related to epidermal Langerhans cells.
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PMID:A distinctive cutaneous malignant neoplasm expressing the Langerhans cell phenotype. Synchronous occurrence with B-chronic lymphocytic leukemia. 388 25

The existence of HLA-DR/Ia-like antigen (Ia)-bearing cells of the mononuclear phagocyte system, macrophages (Mac), and/or interdigitating cells (IDC), in the normal kidney is controversial. If present, such cells may be important in renal transplant rejection. We performed enzyme histochemistry using alpha-naphthyl acetate/butyrate esterases (alpha NAE, alpha NBE), 5'-nucleotidase (5'N), acid phosphatase (AcP), alkaline phosphatase (AlkP), and ATPase (ATP) as well as immunoperoxidase staining for Ia and lectin binding (Ulex europaeus I; UEA) on plastic-embedded tissue sections of normal kidneys and rejected renal allografts. Plastic embedding provides clear visualization of histologic detail and allows specific identification of immunoperoxidase-stained cells. Mac and IDC (shown to be Ia+, alpha NAE+, AcP+, ATP+ in other sites) could not be demonstrated in normal renal interstitium. IDC and Mac were not generally identified in normal mesangium, although they could not be altogether distinguished from Ia+ endothelial cells. Focal mesangial staining for alpha NAE but not alpha NBE was present. Rejected kidneys showed increased numbers of alpha NAE+ cells in glomeruli. These cells were frequently Ia negative and often appeared to be blood monocytes present in capillary lumens. Peritubular capillaries and glomerular endothelium stained strongly for UEA, 5'N, and Ia. Our results suggest that previous reports of the presence of IDC in renal tissue on the basis of staining for Ia on frozen tissue may be due to staining of compressed or obliquely sectioned vascular structures that were not adequately visualized.
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PMID:Monocyte/macrophage derived cells in normal and transplanted human kidneys. 389 Nov 75

Changes occurring in a population of Langerhans cells (LC) were investigated using human gingival epithelium maintained in organ culture. The enzyme adenosine-5'-triphosphatase together with the surface antigens HLA-DR and T6 were used to identify and quantitate these cells. An exponential fall in the expression of each of these markers was seen to occur with increasing incubation time, suggesting that a true loss of these cells is in fact occurring. HLA-DR antigen is shed from LC at a rate significantly greater than that at which LC are lost. A mathematical model which simulates the behaviour of LC within organ culture is presented, and several implications and possible explanations proposed.
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PMID:Loss of Langerhans cells from gingival tissue maintained in organ culture. 615 91

The distribution of Langerhans cells (LCs) in human corneal and conjunctival epithelial sheets was investigated by histochemical, immunofluorescence, and immuno-electron microscopic methods. The LCs stained positive with ATPase and with antibodies to HLA-DR antigen and were negative to DOPA-oxidase. Human conjunctiva showed 250 to 300 LCs/mm2 compared to 15 to 20/mm2 in the peripheral third of the corneal epithelium, approximately similar of LCs were present in Lewis rat, fewer cells in guinea pigs and mice, and no detectable cells in the chick.
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PMID:Langerhans cells in the normal conjunctiva and peripheral cornea of selected species. 617 Jun 2


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