Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.4.4 (adenosine deaminase)
 5,136 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Models of rats with iron deficiency anemia (IDA) were established, and changes in development of the thymus and spleen and adenosine deaminase (ADA) activity in them studied. The size and weight of the thymus in IDA rats were decreased, which suggested that the development of the thymus was lowered; but the size and weight of the spleen in IDA group were increased. ADA activities of the thymus and the spleen in IDA rats were significantly decreased by 32.98% and 25.89%, respectively. One week after iron supplement, the weight of the thymus and spleen in IDS group returned to normal level (P greater than 0.05), and their ADA activity also significantly increased, but ADA activity of the thymus tissue did not return to the normal. The results suggested that the changes in ADA activity of the thymus and spleen tissues were related to iron deficiency. Stepwise regression analysis suggested that there was a negative correlation between ADA activity of the thymus tissue and FEP/Hb ratio.
Hua Xi Yi Ke Da Xue Xue Bao 1990 Mar
PMID:[Influence of iron deficiency anemia on development of thymus and spleen and adenosine deaminase activity in rats]. 236 44

The study of genetic polymorphism of adenosine deaminase (ADA) has not been reported in China and abroad. The target DNA was amplified by PCR. The amplified product was then digested with Pst I, and run on a 9% polyacrylamide gel electrophoresis and followed by AgNO3 staining. ADA polymorphism, was determined in 80 unrelated individuals Chinese Han polulation. Five alleles and 14 genotypes were detected. The gene frequencies were ADA * 10 = 0.15625, ADA * 11 = 0.31875, ADA * 12 = 0.23750, ADA * 13 = 0.23125 and ADA * 14 = 0.05625 respectively. The heterozygosity was 0.8 and the discrimination power (DP) was 0.89. The distribution of observed genotypes frequencies was in good agreement with the Hardy-Weinberg law. The study of three families demonstrated that this locus was inherited according to Mendelian law. The technique is quite simple, fast, extremely sensitive, and it does not require the use of radioisotopes. This new method may be used for the forensic identification and paternity testing.
Hua Xi Yi Ke Da Xue Xue Bao 1998 Mar
PMID:[A study of the adenosine deaminase polymorphism in a Chinese Han population by the PCR method]. 1068 81