Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.4.4 (
adenosine deaminase
)
5,136
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The most common type of RNA editing in metazoans is the deamination of adenosine into inosine (A-to-I) catalyzed by the
adenosine deaminase
acting on the RNA (ADAR) family of proteins. The deletion or dysfunction of ADAR enzymes in higher eukaryotes can affect the efficiency of substrate editing and cause neurological disorders. However, the information concerning A-to-I RNA editing and ADAR members in the silkworm,
Bombyx mori
(BmADAR), is limited. In this study, a first molecular comprehensive cloning and sequence analysis of
BmADAR
transcripts was presented. A complete open reading frame (ORF) (
BmADARa
) was obtained using RT-PCR and RACE and its expression pattern, subcellular localization and A-to-I RNA-editing function on the silkworm
synaptotagmin I
(
BmSyt I
) were investigated. Subcellular localization analysis observed that
BmADARa
was mainly localized in the nucleus. To further study the A-to-I RNA-editing function of
BmADARa
, BmSyt I-pIZ-EGFP was constructed and co-transfected with BmADARa-pIZ-EGFP into BmN cells. The result demonstrates that BmADARa can functionally edit the specific site of
BmSyt I.
Taken together, this study not only provides insight into the function of the first ADAR enzyme in
B. mori
, but also lays foundations for further exploration of the functional domain of BmADARa and its editing substrates and target sites.
...
PMID:Expressional Localization and Functionally Identifying an RNA Editing Enzyme BmADARa of the Silkworm
Bombyx mori
. 3280 97
