Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.4.4 (
adenosine deaminase
)
5,136
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
On the basis of early study on effects of retinoic acid (RA) on the differentiation of mouse lymphocytic leukemia-lymphoma cell strain (SACIIB 2), further research has been performed by studying the effects of retinoic acid on the human T lymphocytic leukemia cell line CCRF-CEM(CEM). The results showed that the growth of CEM cells was inhibited by RA at a concentration of 10 mumol/L. The activity of alpha-naphthyl acetate esterase (ANAE) and the percentage of CD3 positive cells rose after 10 days' RA treatment but the E rosette forming cells didn't increase. The activity of purine nucleoside phosphorylase (PNP) of the treated CEM cells increased significantly without change in activity of
adenosine deaminase
(
ADA
). The expression of terminal deoxynucleotidyl transferase (TdT) was also reduced to some degree. The analysis of
lactic acid dehydrogenase
(
LDH
) isoenzyme showed that the activity of LDH3 increased after RA treatment but without LDH1 and LDH2 expression. The results indicate that RA can induce CEM cells to differentiate.
...
PMID:[Retinoic acid induces differentiation of human T lymphocytic leukemia CCRF-CEM cells]. 817 76
Examination of the pleural fluid is useful in establishing the etiology of a pleural effusion. Transudative pleural effusions can be differentiated from exudative pleural effusions by measuring the levels of protein and
lactic acid dehydrogenase
in the pleural fluid and serum. If a patient clinically appears to have a transudative pleural effusion, but the pleural fluid meets exudative criteria, demonstration that the albumin levels is more than 1.2 gm/dl higher in the serum than in the pleural fluid provides evidence that the effusion is transudative. The gross appearance of the pleural fluid should always be noted. Other tests that routinely should be obtained on exudative pleural fluids are Gram stain and cultures, cell counts and differential, glucose, amylase,
lactic acid dehydrogenase
, cytology, and a marker for tuberculous pleuritis. The diagnosis of tuberculous pleuritis is strongly suggested by a pleural fluid
adenosine deaminase
level above 45 IU/L or a gamma interferon level above 3.7 U/ml.
...
PMID:Useful tests on the pleural fluid in the management of patients with pleural effusions. 1040 95
