Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.4.4 (
adenosine deaminase
)
5,136
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Adenosine deaminase is found primarily in the cytoplasm of many cell types. In the human erythrocyte, about 30 per cent of the total
adenosine deaminase
activity is membrane associated, and about two-thirds of this is inactivated by treatment of intact erythrocytes with the nonpenetrating reagent diazotized sulfanilic acid, without affecting lactate dehydrogenase, a soluble cytoplasmic enzyme. This indicates that within the cell membranes, the catalytic site of about two-thirds of the
adenosine deaminase
faces the external medium, i.e., ecto
adenosine deaminase
. Localization of
adenosine deaminase
activity at the cell membrane is demonstrated directly by electron microscopy by use of the substrate
6-Chloropurine
ribonucleoside, which is dechlorinated by
adenosine deaminase
to produce Cl-, which is precipitated at its locus of formation by added Ag+, and the precipitated AgCl converted into the electron dense Ag0 upon exposure to light. From the Hydropathic Profile of the amino acid sequence of
adenosine deaminase
it is evident that there are two hydrophobic domains of sufficient length to span a biological membrane, and it is proposed that these domains could function to anchor the enzyme to the membrane. The importance of
adenosine deaminase
is indicated by the fatal immuno-deficiency which results from untreated genetic adenosine deaminase deficiency. It may be important to determine whether the amount of ecto
adenosine deaminase
activity is better suited to assess the clinical status of
adenosine deaminase
deficient patients that the currently used total cellular enzyme activity.
...
PMID:Ecto-enzyme activity of human erythrocyte adenosine deaminase. 277 Jul 11
