Gene/Protein Disease Symptom Drug Enzyme Compound
Pivot Concepts:   Target Concepts:
Query: EC:3.5.4.4 (adenosine deaminase)
 5,136 document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)

Two children with acute lymphoblastic leukemia (ALL), whose lymphoblasts lacked terminal deoxynucleotidyl transferase (TdT) by both enzyme and fluorescent antibody assay, responded poorly or not at all to vincristine and prednisone. Both patients had high presenting white counts and mixed L1-L2 morphology. Lymphoblasts from one patient, an adolescent boy with a mediastinal mass, possessed surface membrane receptors for sheep red cells (E) and for complement (EAC) and had elevated adenosine deaminase activity (ADA). Lymphoblasts from a 2.5-yr-old boy without a mediastinal mass did not form E or EAC rosettes and did not express the la-like antigen or carry surface immunoglobulin. The poor response to therapy and absence of TdT were associated with a lymphoblast phenotype suggestive of a highly differentiated T-cell-derived line in one instance and an undifferentiated cell in the other instance. It is postulated that absence of TdT may predict poor therapeutic efficacy of vincristine and prednisone in acute lymphoblastic leukemia in childhood. The absence of TdT may correlate with other developmental characteristics of lymphoblasts, such as altered function or low numbers of glucocorticoid receptors or resistance to lysis by steroid drugs. Determination of many parameters of lymphoblast phenotype at diagnosis to characterize the nature of the malignant cells more precisely may ultimately enhance our understanding of, and improve therapy for, the group of leukemic children who fail to respond to standard regimens.
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PMID:Absence of terminal transferase may predict failure of remission induction in childhood ALL. 657 97

The leukemic cells in chronic lymphatic leukemia (CLL) patients have been studied prior to theory with a panel of immunological markers. Cells were assayed for the presence of receptors for sheep erythrocytes (E active and total rosettes), C3d component of complement (EAC rosettes), mouse erythrocytes (M rosettes), some of them also for surface membrane immunoglobulins (SmIg). In vitro 24 h cultures without mitogen (detection of spontaneous DNA synthesis) or 72 h cultures with phytohemagglutinin (PHA) were also performed. These conventional immunological markers and functional lymphocyte characteristics have been correlated with enzyme activities of adenosine deaminase (ADA) and purine nucleoside phosphorylase (PNP). Electrophoretic patterns of radiolabeled proteins under denaturing conditions (SDS-PAGE) have also been determined in some patients of this group. Phenotypic surface characterization of blood elements of all CLL patients studied revealed their B origin, with increased values of EAC rosette forming cells and especially increased values of M rosette forming cells. Significantly decreased values of both, ADA and PNP, were found in all the cases. Electrophoretic patterns of radiolabeled surface proteins from cells of CLL patients were essentially similar within the group with characteristically strongly radiolabeled glycoproteins gp44--HLA heavy chain and glycoproteins gp29, gp35--Ia-like or HLA-DR antigen.
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PMID:Some immunological and biochemical markers in chronic lymphatic leukemia patients. 681 48

Layered double hydroxides (LDHs) were investigated as cordycepin delivery nanocarriers for the first time in this study. Negatively charged biomolecule-cordycepin was intercalated in the gallery spaces of [Mg-Al-NO(3)] as the charge-compensating species, which was confirmed by the results of XRD, FT-IR, TEM, CZE and electrophoretic mobility. Cell experiment suggested that the new bio-LDH nanohybrid could prevent cordycepin decomposition by adenosine deaminase. This new formulation could possibly be used as a novel form cordycepin intravenous injection.
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PMID:Synthesis and properties of cordycepin intercalates of Mg-Al-nitrate layered double hydroxides. 1688 16

Two general and reliable fluorescence sensors were proposed in this work utilizing aptamer DNA-templated silver nanoclusters (Ag NCs). Both DNA-AgNCs could be used for label-free detecting of ATP with the limits of detection of 0.44 and 0.65mM. One of them was further applied to monitor the activity of adenosine deaminase (ADA). In our effort to elucidate the light-up mechanism, we studied a total of six Ag NCs prepared by different DNA sequences, and found that they showed different sensitivity to ATP. Both BT3T3- and BT3T3(R)-templated Ag NCs were chose to make particular studies by UV-vis, TEM, fluorescence, and TCSPC methods. The results showed that when DNA-Ag NCs was kept for 1.5h and presented a strong fluorescence, the addition of ATP failed to cause a large change of fluorescence intensity; on the contrary, after Ag NCs was kept for 24h and emitted a weak fluorescence, adding ATP was able to result in the large fluorescence enhanced of 43 and 33 times for BT3T3- and BT3T3(R)-templated Ag NCs, respectively. The possible mechanism was also suggested that ATP binding to aptamer segment of template induced the change of the DNA secondary structure, which made the aggregated Ag nanoparticles disperse into Ag NCs with an average diameter of about 2nm that were responsible for the large fluorescence increase. Moreover, ATP could protect the fluorescence intensity of BT3T3(R)-templated Ag NCs from quenching for at least 9h.
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PMID:The aptamer DNA-templated fluorescence silver nanoclusters: ATP detection and preliminary mechanism investigation. 2758 6