Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.4.4 (
adenosine deaminase
)
5,136
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
A novel strategy for the preparation of in-column
adenosine deaminase
(
ADA
) microreactor and rapid screening of enzyme inhibitors in natural extracts was demonstrated. In this approach,
ADA
was encapsulated in anionic polyelectrolyte alginate that was immobilized on the surface of fused-silica capillary via ionic binding technique with cationic polyelectrolyte polyethylenimine (PEI). On-line enzyme inhibition study was performed by capillary electrophoresis (CE). The substrate and product were baselined separated within 75s. The enzyme activity was determined by the quantification of peak area of the product.
Enzyme inhibition
can be read out directly from the reduced peak area of the product in comparison with a reference electropherogram obtained in the absence of any inhibitor. The inhibition percentage was used to evaluate relative activity of
ADA
microreactor. A known
ADA
inhibitor, erythro-9-(2-hydroxy-3-nonyl) adenine (EHNA) was employed as a model compound for the validation of the inhibitor screening method, and the screening of
ADA
inhibitor in 19 traditional Chinese herbal medicines was performed.
...
PMID:Immobilized capillary adenosine deaminase microreactor for inhibitor screening in natural extracts by capillary electrophoresis. 2080 14
