Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.4.4 (
adenosine deaminase
)
5,136
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
adenosine deaminase
(
ADA
) locus appears to be under complex transcriptional control since levels of
ADA
enzyme activity vary greatly between different tissues and stages of development. Evidence that a trans-acting factor may be involved with the regulation of this locus came from previous experiments where fusion of
ADA
-negative human JEG cells and mouse
ADA
-positive cells led to the trans-activation of human
ADA
in a hybrid nucleus. Here, we demonstrate that the near euploid mouse embryo fibroblast cell line,
CAK
, also lacks detectable
ADA
enzyme activity due to altered gene regulation. We further demonstrate that
ADA
in
CAK
cells is not amenable to activation by somatic cell fusion. Following treatment with 5-azacytidine and Xyl-A selection (for
ADA
), however,
CAK
clones were obtained that stably express the
ADA
gene. Molecular analysis of the parental
CAK
cells and the
ADA
-positive derivative clones demonstrated that both 5' and 3' regions of the
ADA
gene had become hypomethylated in the ADA+ clones. We conclude that methylation is another element involved with the transcriptional control of the
ADA
gene and that
ADA
might serve as a useful model for studying the interaction of cis- and trans-acting regulational elements.
...
PMID:Hypomethylation and ADA gene expression in mouse CAK cells. 246 55
