Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.52 (
PNGase F
)
1,527
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Our previous studies have shown that the
DAN
gene product possesses an ability to revert phenotypes of transformed rat fibroblasts and represents a candidate tumour suppressor gene for neuroblastoma. In the present study, characterisation of
DAN
was carried out using rat fibroblast 3Y1 cells and their
DAN
-overexpressor counterparts (S-9). The N-terminal region of
DAN
(amino acids 1-24) was highly hydrophobic and
DAN
protein was found to be secreted into the culture medium. When
DAN
was treated with
PNGase F
, a enzyme that cleaves most N-linked carbohydrate residues, the mobility of both cytoplasmic and secreted
DAN
was increased in SDS-polyacrylamide gel electrophoresis, suggesting
DAN
is N-glycosylated, irrespective of its localisation. When partially purified,
DAN
was able, when added to the culture, to suppress DNA synthesis of Rous sarcoma virus-transformed 3Y1 cells, which lack the expression of
DAN
.
...
PMID:A product of DAN, a novel candidate tumour suppressor gene, is secreted into culture medium and suppresses DNA synthesis. 951 39
