Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.52 (
PNGase F
)
1,527
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
myelin-associated glycoprotein
(
MAG
) has an extracellular domain containing five sequences which are homologous to the immunoglobulin-fold motif. Adhesive interactions mediated by the
MAG
extracellular domain are involved in the development of the myelin sheath. The
MAG
cDNA has been modified to introduce a stop codon immediately before the transmembrane domain. Expression of the modified cDNA in insect cells and murine NIH-3T3 cells resulted in secretion of the soluble
MAG
extracellular domain. Treatment of soluble
MAG
with
glycopeptidase
F and endoglycosidase H showed significant differences in glycosylation for the insect and mammalian cell-expression systems. The soluble form of
MAG
has been purified from insect-cell supernatants by adsorption to a lentil-lectin support. The soluble
MAG
will provide a powerful new approach for studies of
MAG
-adhesive interactions during brain development.
...
PMID:Synthesis of soluble myelin-associated glycoprotein in insect and mammalian cells. 247 45
