Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.52 (
PNGase F
)
1,527
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
The
glycopeptidase
GCP and its homologue proteins are conserved and essential for survival of bacteria. The
ygjD
gene (Glycopeptidase homologue) was cloned from
Vibrio harveyi
strain
SF-1
. The gene consisted of 1,017 bp, which encodes a 338 amino acid polypeptide. The nucleotide sequence similarity of the
ygjD
gene with that of
V. harveyi
FDAARGOS 107 was 95%. The
ygjD
gene also showed similarities of 68%, 67% and 50% with those of
Salmonella enterica
,
Escherichia coli
and
Bacillus cereus
. The
ygjD
gene was expressed in
E. coli
BL21 (DE3) and the recombinant YgjD was purified by Ni
2+
affinity chromatography column. The purified YgjD showed a specific 37 kDa band on sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) and exhibited protease activities of 59,000 units/mg, 53,700 units/mg and 8,100 units/mg, respectively, on N-Acetyl-L-tyrosine ethyl ester monohydrate (ATEE), N-Benzoyl-L-tyrosine ethyl ester (BTEE) and N-Benzoyl-DL-arginine-4-nitroanilide hydrochloride (BAPNA) substrates. When the conserved amino acids of His
111
, Glu
113
and His
115
in the YgjD were replaced with alanine, respectively, the protease activities of the mutants were partly decreased. The two conserved His
111
and His
115
of YgjD were mutated and the protein lost the protease activity, which implied that the two amino acid played very important roles in maintaining its protease activity. The addition of the purified YgjD to the culture medium of
V. harveyi
strain
SF-1
can effectively promote the bacteria growth. These results indicated that the protease activities may be involved in the survival of bacteria.
...
PMID:Expression and protease characterization of a conserved protein YgjD in
Vibrio harveyi
. 3247 34
