Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.5 (
urease
)
7,257
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
In 2015,
Bacillus paralicheniformis
was separated from
B. licheniformis
on the basis of phylogenomic and phylogenetic studies, and
urease
activity was reported as a phenotypic property able to differentiate between the two species. Subsequently, we have found that the
urease
activity of
B. paralicheniformis
is strain-specific, and does not reliably discriminate between species, as strains having the same
urease
gene cluster were identified in
B. licheniformis
and
B. sonorensis
, the closest relatives of
B. paralicheniformis
. We developed a multilocus sequence typing scheme using eight housekeeping genes,
adk, ccpA, glpF,
gmk
, ilvD, pur, spo0A
, and
tpi
to clearly identify
B. paralicheniformis
from closely related
Bacillus
species and to find a molecular marker for the rapid identification of
B. paralicheniformis
. The scheme differentiated 33
B. paralicheniformis
strains from 90 strains formerly identified as
B. licheniformis
. Among the eight housekeeping genes,
spo0A
possesses appropriate polymorphic sites for the design of a
B. paralichenofomis
-specific PCR primer set. The primer set designed in this study perfectly separated
B. paralicheniformis
from
B. licheniformis
and
B. sonorensis
.
...
PMID:Urease Characteristics and Phylogenetic Status of
Bacillus paralicheniformis
. 3039 48
