Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.4 (
deaminase
)
5,113
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Polyamines (PAs) regulate growth in plants and modulate the whole plant life cycle. They have been associated with different abiotic and biotic stresses, but little is known about the molecular regulation involved. We quantified gene expression of PA anabolic and catabolic pathway enzymes in tomato (
Solanum lycopersicum
cv. Ailsa Craig) leaves under heat versus cold stress. These include
arginase
1
and
2
,
arginine decarboxylase 1
and
2
,
agmatine iminohydrolase
/
deiminase 1
,
N-carbamoyl putrescine
amidase
, two
ornithine decarboxylases
, three
S-adenosylmethionine decarboxylases
, two
spermidine synthases
;
spermine synthase
;
flavin-dependent polyamine oxidases
(
SlPAO4-like
and
SlPAO2
) and
copper dependent amine oxidases
(
SlCuAO
and
SlCuAO-like
). The spatiotemporal transcript abundances using qRT-PCR revealed presence of their transcripts in all tissues examined, with higher transcript levels observed for
SAMDC1
,
SAMDC2
and
ADC2
in most tissues. Cellular levels of free and conjugated forms of putrescine and spermidine were found to decline during heat stress while they increased in response to cold stress, revealing their differential responses. Transcript levels of
ARG2
,
SPDS2
, and
PAO4-like
increased in response to both heat and cold stresses. However, transcript levels of
ARG1/2
,
AIH1
,
CPA
,
SPDS1
and
CuAO4
increased in response to heat while those of
ARG2, ADC1,2,
ODC1
, SAMDC1,2,3, PAO2
and
CuPAO4-like
increased in response to cold stress, respectively. Transcripts of
ADC1,2
,
ODC1
,2
, and
SPMS
declined in response to heat stress while
ODC2
transcripts declined under cold stress. These results show differential expression of PA metabolism genes under heat and cold stresses with more impairment clearly seen under heat stress. We interpret these results to indicate a more pronounced role of PAs in cold stress acclimation compared to that under heat stress in tomato leaves.
...
PMID:Polyamines and Their Biosynthesis/Catabolism Genes Are Differentially Modulated in Response to Heat Versus Cold Stress in Tomato Leaves (
Solanum lycopersicum
L.). 3270 44
