Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Pivot Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Target Concepts:
Gene/Protein
Disease
Symptom
Drug
Enzyme
Compound
Query: EC:3.5.1.4 (
deaminase
)
5,113
document(s) hit in 31,850,051 MEDLINE articles (0.00 seconds)
Among 29 strains of zygomycetes screened for serine carboxypeptidases, Absidia zychae NRIC 1199 showed the highest enzyme production. Two serine carboxypeptidases,
CPZ
-1 and
CPZ
-2, were purified to a homogeneous state from an extract of koji culture of A. zychae NRIC 1199. Purified
CPZ
-1 and
CPZ
-2 showed similar properties except the isoelectric point (pI); The pI of
CPZ
-1 and
CPZ
-2 were 4.50 and 4.65, respectively. The molecular weights of the
CPZ
-1 and
CPZ
-2 were 48,000 by SDS-PAGE and gel filtration. Among the proteinase inhibitors tested, phenylmethylsulfonyl fluoride and monoiodoacetic acid strongly inhibited the enzyme activity. The optimum pHs of
CPZ
-1 and
CPZ
-2 were 4.2 towards Z-Glu-Tyr. It is shown that the substrate specificities of
CPZ
-1 and
CPZ
-2 were dependent on the presence of bulky amino acid residues in the penultimate position (P1) for the small Z-peptides. However, in spite of the presence of Gly, Asp, Arg, or Pro in the P1 position, oligopeptides were hydrolyzed rapidly.
CPZ
-1 and
CPZ
-2 had not only carboxypeptidase but also carboxyamidase and
amidase
activities, and acted preferentially as a carboxyamidase for C-terminal amidated peptides. The hydrophobicity of P2 and P3 positions and the bulkiness of P1 and P'1 positions of the substrate may be important for carboxyamidase and
amidase
activities.
...
PMID:Purification and characterization of serine carboxypeptidases from Absidia zychae. 776 58
